Purification of Larvicidal Protein from Bacillus Sphaericus 1593

Narasu, Lakshmi M and Gopinathan, KP (1986) Purification of Larvicidal Protein from Bacillus Sphaericus 1593. In: Biochemical and Biophysical Research Communications, 141 (2). pp. 756-761.

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Abstract

Coat proteins from the spores of Bacillus sphaericus 1593 were separated by preparative polyacrylamide gel electrophoresis. Neutralising antibodies were raised against a single protein band exhibiting toxicity to mosquito larvae. IgG was purified and coupled to CNBr-activated Sepharose 4B to be used as an immunoaffinity matrix. The larvicidal protein was purified to electrophoretic homogeneity using this immunoaffinity column. The single protein species resolved into four peptides of molecular weights 42.6, 44.1, 50.7 and 51.3 KDa on polyacrylamide gel electrophoresis under denaturing conditions. This protein contained 12% carbohydrates. The purified protein exhibited an LC50 value of 8.3$\pm$1.6 ng/ml when tested against early third instar larvae of the mosquito Culex pipiens var quinquefasciatus.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Additional Information:	Copyright for this article belongs to Elsevier.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	05 Aug 2008
Last Modified:	19 Sep 2010 04:48
URI:	http://eprints.iisc.ac.in/id/eprint/15393

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