Recombinant L and P protein complex of Rinderpest virus catalyses mRNA synthesis in vitro

Gopinath, M and Shaila, MS (2008) Recombinant L and P protein complex of Rinderpest virus catalyses mRNA synthesis in vitro. In: Virus Research, 135 (1). pp. 150-154.

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Abstract

Rinderpest virus belongs to the family of Paramyxoviridae, consisting of non-segmented negative senseRNA viruses. Viral transcription and replication are carried out by theRNAdependentRNApolymerase L protein which functions together with P protein as L–P complex. The exact events triggering the polymerase complex from transcription to replication function is poorly understood. In the present work, an in vitro transcription system has been described with partially purified L–P complex expressed in insect cells and viral genomic RNA. The relative abundance of each species of mRNA synthesized in vitro decreased from the 3 end of the genome to the 5 end similar to their abundance in virus infected cells. Recombinant L–P complex was unable to synthesize leader RNA suggesting the initiation of transcription from gene start site and not at the 3 end of the genome.

Item Type:	Journal Article
Publication:	Virus Research
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Rinderpest virus;In vitro transcription;RNA dependent;RNA polymerase.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	21 Jul 2008
Last Modified:	19 Sep 2010 04:47
URI:	http://eprints.iisc.ac.in/id/eprint/15170

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