Cloning and expression of genes for lysozyme and a 20-kDal protein of colitis bacteriophage

Vasavada, HA and Murthy, Indira and Padayatty, JD (1985) Cloning and expression of genes for lysozyme and a 20-kDal protein of colitis bacteriophage. In: Gene, 34 (1). pp. 9-15.

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Abstract

BamHI fragments of colitis phage DNA were cloned in pBR322 DNA, and the recombinant clones carrying the lysozyme gene were identified by lysozyme activity. The inserted DNA was 1.2 kb long and when expressed in minicells it produced lysozyme and a 20-kDal protein. Colitis-phage-specific mRNAs which hybridized to the insert were 0.5 kb and 0.7 kb long and were translated into lysozyme and a 20-kDal protein, respectively, in a cell-free system derived from rice embryos. They were transcribed as monocistronic mRNAs using the internal promoters present on the inserted DNA.

Item Type:	Journal Article
Publication:	Gene
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	16 Jul 2008
Last Modified:	19 Sep 2010 04:47
URI:	http://eprints.iisc.ac.in/id/eprint/14979

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