Savithri, Handanahal S and Munshi, Sanjeev K and Suryanarayana, Sankuratri and Divakar, Soundar and Murthy, Mathur RN (1987) Stability of belladonna mottle virus particles: the role of polyamines and calcium. In: Journal of General Virology, 68 (6). pp. 1533-1542.
![]() |
PDF
1987_JGV_V68_pp1533-1542.pdf Restricted to Registered users only Download (5MB) | Request a copy |
Abstract
The stability of belladonna mottle virus (BDMV) has been studied with respect to elevated pH and to freezing and thawing. BDMV, purified by a modified procedure, was stable at alkaline pH, in contradiction to earlier reports. This difference in the stability could be attributed to the presence of 90 to 140 molecules of spermidine, 20 to 50 molecules of putrescine and 500 to 900 calcium ions in each virus particle. The polyamines could be easily exchanged with other cations such as potassium or caesium and this resulted in a loss of particle stability. These cations may therefore play a role in maintaining the integrity of particle structure. The formation of empty protein shells as a result of freezing and thawing BDMV particles parallels earlier observations on turnip yellow mosaic virus particles.
Item Type: | Journal Article |
---|---|
Publication: | Journal of General Virology |
Publisher: | Society for General Microbiology |
Additional Information: | Copyright for this article belongs to the Society for General Microbiology |
Keywords: | BDMV;capsid stability;polyamines |
Department/Centre: | Division of Biological Sciences > Biochemistry |
Date Deposited: | 09 May 2008 |
Last Modified: | 19 Sep 2010 04:44 |
URI: | http://eprints.iisc.ac.in/id/eprint/13898 |
Actions (login required)
![]() |
View Item |