Isolation of a cytochrome P-450e gene variant and characterization of its $5^{\prime}$ flanking sequences

Rangarajan, PN and Ravishankar, H and Padmanaban, G (1987) Isolation of a cytochrome P-450e gene variant and characterization of its $5^{\prime}$ flanking sequences. In: Biochemical and Biophysical Research Communications, 144 (1). pp. 258-263.

PDF 1987_BBRC_V144_pp258-263.pdf Restricted to Registered users only Download (414kB) | Request a copy

Abstract

A cytochrome P-450e gene variant has been isolated from the rat liver genomic library. It is a typical e gene clone but unique in having b-like single base substitutions at specific sites in the $5^{\prime}$ flanking region. It also appears to have certain additional restriction sites in the introns. When compared with the cytochrome P-450b gene, the e gene has some of the repetitive motifs interrupted in the $5^{\prime}$ flanking region. In addition, this region is characterized by the presence of alternating pyrimidine-purine stretch, steroid hormone regulatory elements, consensus eukaryotic enhancer sequence and sequences involved in general amino acid regulation.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier
Additional Information:	Copyright of this article belongs to Elsevier.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	05 May 2008
Last Modified:	19 Sep 2010 04:44
URI:	http://eprints.iisc.ac.in/id/eprint/13848

Actions (login required)

View Item