Differential effects of cycloheximide on rat liver cytochrome P-450 gene transcription in the whole animal and hepatoma cell culture

Bhat, Jayarama G and Rangarajan, PN and Padmanaban, G (1987) Differential effects of cycloheximide on rat liver cytochrome P-450 gene transcription in the whole animal and hepatoma cell culture. In: Biochemical and Biophysical Research Communications, 148 (3). pp. 1118-1123.

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Abstract

The induction of cytochrome P 450 (c+d) messenger RNAs in rat liver by 3-Methyl cholanthrene follows a biphasic pattern. Administration of cycloheximide blocks the induction of cytochrome P 450 (c+d) messenger RNAs by 3-methylcholanthrene as well as cytochrome P 450 (b+e) messenger RNAs by phenobarbitone. Transcription of these messenger RNAs in isolated nuclei is also blocked by cycloheximide administration. Thus, cycloheximide not only fails to mimic the superinduction effects reported in hepatoma cell cultures, but actually blocks the specific transcription process. Exogenous hemin, while counteracting the effects of $CoCl_2$ (heme biosynthetic inhibitor) on cytochrome (c+d) messenger RNA induction by the hydrocarbon, fails to counteract the effects of cycloheximide. It is suggested that a positive labile transcription factor is involved in the regulation of cytochrome P 450 gene expression in vivo.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Elsevier Inc
Additional Information:	Copyright of this article belongs to Academic Press, Inc.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	10 Mar 2008
Last Modified:	19 Sep 2010 04:43
URI:	http://eprints.iisc.ac.in/id/eprint/13282

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