Inhibition of In Vitro Transcription by Adenosine Antibodies

Vaishnav, YN and Antony, A (1990) Inhibition of In Vitro Transcription by Adenosine Antibodies. In: Molecular Immunology, 27 (11). pp. 1113-1118.

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Abstract

Antibodies against adenosine markedly inhibited in vitro transcription in isolated BHK 21 nuclei in a dose-dependent manner. The inhibition was specific as it could be completely reversed by the addition of homologous hapten. Addition of RNA at low concentration reversed the inhibition, whereas excess DNA did not have any effect. Adenosine antibodies also inhibited in vitro transcription with calf thymus DNA and E. coli RNA polymerase. Antibodies that react with DNA but not with RNA such as anti-dpA, anti-dpC and anti-DNA failed to inhibit in vitro transcription in isolated nuclei as well as with calf thymus DNA and E. coli RNA polymerase. The results strongly indicate that the binding of adenosine antibodies to RNA is responsible for the inhibition of transcription.

Item Type:	Journal Article
Publication:	Molecular Immunology
Publisher:	Elsevier Science Ltd
Additional Information:	Copyright of this article belongs to Elsevier Science Ltd.
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	08 Nov 2007
Last Modified:	19 Sep 2010 04:41
URI:	http://eprints.iisc.ac.in/id/eprint/12444

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