Receptor-Mediated Gene Delivery Approach Demonstrates the Role of 5′-Proximal DNA Region in Conferring Phenobarbitone Responsiveness to CYP2B2 Gene in Rat Liver in Vivo

Mani, SA and Harish, S and Vathsala, PG and Rangarajan, PN and Padmanaban, G (2000) Receptor-Mediated Gene Delivery Approach Demonstrates the Role of 5′-Proximal DNA Region in Conferring Phenobarbitone Responsiveness to CYP2B2 Gene in Rat Liver in Vivo. In: Biochemical and Biophysical Research Communications, 268 (3). pp. 734-739.

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Abstract

The phenobarbitone (PB) responsiveness of the 5′-proximal region of the CYP2B1/B2 gene was examined in detail with plasmid DNA constructs containing G-free cassette as reporter, using in vivo targeting of the same DNA constructs into rat liver as galactosylated-polylysine complexes. The contribution of the proximal region (−1 to −179 bp) and the positive element (−69 to −98 bp) identified earlier in this laboratory to PB responsiveness was assessed. The results obtained on PB treatment of rats subjected to receptor-mediated gene delivery to liver were conclusive and dramatic, with the control (saline-treated) rats manifesting very little expression of the reporter, reflecting the in vivo picture of CYP2B1/B2 gene expression. The positive element conferred PB responsiveness to homologous and heterologous promoters. Deletion of the positive element led to elimination of PB response. The entire −179 bp region was significantly more effective in responding to PB treatment than the region up to −98 bp, both containing one copy of the positive element. Thus, the positive element and its flanking sequences in the 5′-proximal region are involved in conferring PB responsiveness to the CYP2B1/B2 gene.

Item Type:	Journal Article
Publication:	Biochemical and Biophysical Research Communications
Publisher:	Academic Press
Additional Information:	Copyright of this article belongs to Academic Press.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	15 Oct 2007
Last Modified:	19 Sep 2010 04:39
URI:	http://eprints.iisc.ac.in/id/eprint/12008

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