Mani, SA and Harish, S and Vathsala, PG and Rangarajan, PN and Padmanaban, G (2000) Receptor-Mediated Gene Delivery Approach Demonstrates the Role of 5′-Proximal DNA Region in Conferring Phenobarbitone Responsiveness to CYP2B2 Gene in Rat Liver in Vivo. In: Biochemical and Biophysical Research Communications, 268 (3). pp. 734-739.
![]() |
PDF
Receptor-mediated_gene_delivery_approach.pdf Restricted to Registered users only Download (155kB) | Request a copy |
Abstract
The phenobarbitone (PB) responsiveness of the 5′-proximal region of the CYP2B1/B2 gene was examined in detail with plasmid DNA constructs containing G-free cassette as reporter, using in vivo targeting of the same DNA constructs into rat liver as galactosylated-polylysine complexes. The contribution of the proximal region (−1 to −179 bp) and the positive element (−69 to −98 bp) identified earlier in this laboratory to PB responsiveness was assessed. The results obtained on PB treatment of rats subjected to receptor-mediated gene delivery to liver were conclusive and dramatic, with the control (saline-treated) rats manifesting very little expression of the reporter, reflecting the in vivo picture of CYP2B1/B2 gene expression. The positive element conferred PB responsiveness to homologous and heterologous promoters. Deletion of the positive element led to elimination of PB response. The entire −179 bp region was significantly more effective in responding to PB treatment than the region up to −98 bp, both containing one copy of the positive element. Thus, the positive element and its flanking sequences in the 5′-proximal region are involved in conferring PB responsiveness to the CYP2B1/B2 gene.
Item Type: | Journal Article |
---|---|
Publication: | Biochemical and Biophysical Research Communications |
Publisher: | Academic Press |
Additional Information: | Copyright of this article belongs to Academic Press. |
Department/Centre: | Division of Biological Sciences > Biochemistry |
Date Deposited: | 15 Oct 2007 |
Last Modified: | 19 Sep 2010 04:39 |
URI: | http://eprints.iisc.ac.in/id/eprint/12008 |
Actions (login required)
![]() |
View Item |