Refinements of the Differential Display Reverse Transcription–Polymerase Chain Reaction Technique: Use of Oligo(dT)-Based Anchored Primers with Escherichia coli Messenger RNA Identifies a Salt-Induced Promoter in the dcw Gene Cluster

Amara, Rama Rao and Satchidanandam, Vijaya (2000) Refinements of the Differential Display Reverse Transcription–Polymerase Chain Reaction Technique: Use of Oligo(dT)-Based Anchored Primers with Escherichia coli Messenger RNA Identifies a Salt-Induced Promoter in the dcw Gene Cluster. In: Analytical Biochemistry, 278 (1). pp. 83-86.

PDF Refinements_of_the_differential_display.pdf Restricted to Registered users only Download (102kB) | Request a copy

Abstract

Differential display reverse transcription PCR (DDRT–PCR)2 is a popular technique used to identify differences in messenger RNA species expressed under different conditions (1–4). The use of this technique in prokaryotic systems has been greatly hampered by the lack of a poly(A) tail at the 39 end of these messages, resulting in the use of random primers to define both ends of the PCR products, in turn leading to lower probability of identifying any differentially expressed gene.

Item Type:	Journal Article
Publication:	Analytical Biochemistry
Publisher:	Academic Press
Additional Information:	Copyright of this article belongs to Academic Press
Keywords:	Molecular Genetics;Biochemistry;Molecular Biophysics;Methods Techniques
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	15 Oct 2007
Last Modified:	20 Feb 2019 06:16
URI:	http://eprints.iisc.ac.in/id/eprint/11989

Actions (login required)

View Item