Saravanan, Matheshwaran and Elango, Kathirvel and Chandrashekaran, Siddamadappa and Anand, Narayanaswamy and Nagaraja, Valakunja (2003) Catechol dioxygenase is an insensitive reporter for transcription in Mycobacterium smegmatis. In: Current Science, 85 (2). pp. 188-191.
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Abstract
Although restriction enzymes are widely distributed in nature, many bacterial genera are yet to be explored for the presence of this important class of enzymes. We have purified and characterized a new type II restriction endonuclease, OfoI from a nonheterocyst cyanobacterium Oscillatoria foreaui. The recognition sequence has been determined by primer extension analysis. The purified enzyme OfoI recognizes and cleaves the palindromic hexanucleotide 5¢-C¯YCGRG-3¢, generating 5¢-protruding ends.
Item Type: | Journal Article |
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Publication: | Current Science |
Publisher: | Indian Academy of Science |
Keywords: | nonheterocystous cyanobacterium Oscillatoria foreaui |
Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
Date Deposited: | 22 Jul 2004 |
Last Modified: | 19 Sep 2010 04:14 |
URI: | http://eprints.iisc.ac.in/id/eprint/1124 |
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