"Plasmo2D": An Ancillary Proteomic Tool to Aid Identification of Proteins from Plasmodium falciparum

Khachane, Amit and Kumar, Ranjit and Jain, Sanyam and Jain, Samta and Banumathy, Gowrishankar and Singh, Varsha and Nagpal, Saurabh and Tatu, Utpal (2005) "Plasmo2D": An Ancillary Proteomic Tool to Aid Identification of Proteins from Plasmodium falciparum. In: Journal of Proteome Research, 4 (6). 2369 -2374.

PDF Plasmo2D-An_Ancillary_Proteomic.pdf Restricted to Registered users only Download (483kB) | Request a copy

Abstract

Bioinformatics tools to aid gene and protein sequence analysis have become an integral part of biology in the post-genomic era. Release of the Plasmodium falciparum genome sequence has allowed biologists to define the gene and the predicted protein content as well as their sequences in the parasite. Using pI and molecular weight as characteristics unique to each protein, we have developed a bioinformatics tool to aid identification of proteins from Plasmodium falciparum. The tool makes use of a Virtual 2-DE generated by plotting all of the proteins from the Plasmodium database on a pI versus molecular weight scale. Proteins are identified by comparing the position of migration of desired protein spots from an experimental 2-DE and that on a virtual 2-DE. The procedure has been automated in the form of user-friendly software called "Plasmo2D". The tool can be downloaded from http://144.16.89.25/Plasmo2D.zip.

Item Type:	Journal Article
Publication:	Journal of Proteome Research
Publisher:	American Chemical Society
Additional Information:	Copyright of this article belongs to the American Chemical Society.
Keywords:	Plasmo2D;Bioinformatics;Proteomics;Malaria heat shock proteins
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	09 Apr 2007
Last Modified:	19 Sep 2010 04:36
URI:	http://eprints.iisc.ac.in/id/eprint/10563

Actions (login required)

View Item