Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus

Kanaujia, Shankar Prasad and Ranjani, Chellamuthu Vasuki and Jeyakanthan, Jeyaraman and Baba, Seiki and Kuroishi, Chizu and Ebihara, Akio and Shinkai, Akeo and Kuramitsu, Seiki and Shiro, Yoshitsugu and Sekar, Kanagaraj and Yokoyama, Shigeyuki (2007) Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communication, 63 (2). pp. 103-105.

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Abstract

The aerobic Gram-positive bacterium Geobacillus kaustophilus is a bacillus species that was isolated from deep-sea sediment from the Mariana Trench. 1,4-Dihydroxy-2-naphthoate (DHNA) synthetase plays a vital role in the biosynthesis of menaquinone (vitamin $K_2$) in this bacterium. DHNA synthetase from Geobacillus kaustophilus was crystallized in the orthorhombic space group $C222_1$, with unit-cell parameters a = 77.01, b = 130.66, c = 131.69 \AA. The crystal diffracted to a resolution of 2.2 \AA. Preliminary studies and molecularreplacement calculations reveal the presence of three monomers in the asymmetric unit.

Item Type:	Journal Article
Publication:	Acta Crystallographica Section F: Structural Biology and Crystallization Communication
Publisher:	International Union of Crystallography
Additional Information:	Copyright of this article belongs to International Union of Crystallography.
Department/Centre:	Division of Interdisciplinary Sciences > Supercomputer Education & Research Centre Division of Information Sciences (Doesn't exist now) > BioInformatics Centre
Date Deposited:	27 Jun 2007
Last Modified:	19 Sep 2010 04:36
URI:	http://eprints.iisc.ac.in/id/eprint/10246

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