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Isolation and molecular characterization of dengue virus clinical isolates from pediatric patients in New Delhi

Kar, Meenakshi and Nisheetha, Amul and Kumar, Anuj and Jagtap, Suraj and Shinde, Jitendra and Singla, Mohit and Saranya, M and Pandit, Awadhesh and Chandele, Anmol and Kabra, Sushil K and Krishna, Sudhir and Roy, Rahul and Lodha, Rakesh and Pattabiraman, Chitra and Medigeshi, Guruprasad R (2019) Isolation and molecular characterization of dengue virus clinical isolates from pediatric patients in New Delhi. In: INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 84 (S). S25-S33.

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Official URL: https://dx.doi.org/10.1016/j.ijid.2018.12.003

Abstract

Objective: To characterize the in vitro replication fitness, viral diversity, and phylogeny of dengue viruses (DENV) isolated from Indian patients. Methods: DENV was isolated from whole blood collected from patients by passaging in cell culture. Passage 3 viruses were used for growth kinetics in C6/36 mosquito cells. Parallel efforts also focused on the isolation of DENV RNA from plasma samples of the same patients, which were processed for next-generation sequencing. Results: It was possible to isolate 64 clinical isolates of DENV, mostly DENV-2. Twenty-five of these were further used for growth curve analysis in vitro, which showed a wide range of replication kinetics. The highest viral titers were associated with isolates from patients with dengue with warning signs and severe dengue cases. Full genome sequences of 21 DENV isolates were obtained. Genome analysis mapped the circulating DENV-2 strains to the Cosmopolitan genotype. Conclusions: The replication kinetics of isolates from patients with mild or severe infection did not differ significantly, but the viral titers varied by two orders of magnitude between the isolates, suggesting differences in replication fitness among the circulating DENV-2.

Item Type: Journal Article
Additional Information: copyright for this article belongs to ELSEVIER SCI LTD
Keywords: Dengue virus; Growth curve; Phylogenetics; Genotype; Mutations
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Interdisciplinary Research > Centre for Biosystems Science and Engineering
Division of Mechanical Sciences > Chemical Engineering
Depositing User: Id for Latest eprints
Date Deposited: 20 Sep 2019 05:59
Last Modified: 20 Sep 2019 05:59
URI: http://eprints.iisc.ac.in/id/eprint/63495

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