Heterologous expression of P5CS gene in chickpea enhances salt tolerance without affecting yield

Ghanti, Kiran Kumar S and Sujata, KG and Kumar, Vijay BM and Karba, Nataraja N and Reddy, Janardhan K and Rao, Srinath M and Kishor, Kavi PB (2011) Heterologous expression of P5CS gene in chickpea enhances salt tolerance without affecting yield. In: Biologia Plantarum, 55 (4). pp. 634-640.

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Abstract

Vigna Delta(1)-pyrroline-5-carboxylate synthetase (P5CS) cDNA was transferred to chickpea (Cicer arietinum L.) cultivar Annigeri via Agrobacterium tumefaciens mediated transformation. Following selection on hygromycin and regeneration, 60 hygromycin-resistant plants were recovered. Southern blot analysis of five fertile independent lines of T0 and T1 generation revealed single and multiple insertions of the transgene. RT-PCR and Western blot analysis of T0 and T1 progeny demonstrated that the P5CS gene is expressed and produced functional protein in chickpea. T1 transgenic lines accumulated higher amount of proline under 250 mM NaCl compared to untransformed controls. Higher accumulation of Na(+) was noticed in the older leaves but negligible accumulation in seeds of T1 transgenic lines as compared to the controls. Chlorophyll stability and electrolyte leakage indicated that proline overproduction helps in alleviating salt stress in transgenic chickpea plants. The T1 transgenics lines were grown to maturity and set normal viable seeds under continuous salinity stress (250 mM) without any reduction in plant yield in terms of seed mass.

Item Type:	Journal Article
Publication:	Biologia Plantarum
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	Agrobacterium tumefaciens;Cicer arietinum;Delta(1)-pyrroline-5-carboxylate synthetase;NaCl;proline accumulation;RT-PCR; Southern blot;Western blot
Department/Centre:	Others
Date Deposited:	17 Nov 2011 08:50
Last Modified:	17 Nov 2011 08:50
URI:	http://eprints.iisc.ac.in/id/eprint/42051

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