CD and NMR studies on the aggregation of amphotericin-B in solution

Balakrishnan, Rajini A and Easwaran, KRK (1993) CD and NMR studies on the aggregation of amphotericin-B in solution. In: Biochimica et Biophysica Acta (BBA) - Biomembranes, 1148 (2). pp. 269-277.

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Abstract

We report in this paper the aggregation properties of amphotericin-B (amp-B) in solution using CD and 1H-NMR techniques. Our results indicate that the preferred structure of amp-B in dimethylsulfoxide is a monomer at low concentrations (10−4M and below) and a stable dimer at higher concentrations (range 5 · 103 M to 10−2M). In a DMSO/ethanol mixture (1:1 (v/v)), the antibiotic is monomeric, irrespective of the concentration within the range studied. We propose a head-to-tail model based on NMR data. An understanding of the head-to-tail dimer, is, we believe important, particularly in view of the recent report wherein it is proposed that the drug inserts into bilayers as head-to-tail oligomers.

Item Type:	Journal Article
Publication:	Biochimica et Biophysica Acta (BBA) - Biomembranes
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Polyene antifungal antibiotic;NMR, 1H-;Circular dichroism; Aggregation;Amphotericin B.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	02 Feb 2011 07:15
Last Modified:	02 Feb 2011 07:15
URI:	http://eprints.iisc.ac.in/id/eprint/35358

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