ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Perturbation of lipid structures by fluorescent probes

Krishnan, KS and Balaram, P (1975) Perturbation of lipid structures by fluorescent probes. In: FEBS Letters, 60 (2). pp. 419-422.

[img] PDF
Perturbation.pdf
Restricted to Registered users only

Download (295kB) | Request a copy

Abstract

The equilibrium fluorescent probe 1 -aniline-8-naphthalene sulfonate*, has found extensive use in studies of membrane structure. In an entertainingly written review, Radda and Vanderkooi have posed the question, ‘Can fluorescent probes tell us anything about membranes?’ [1]. A great deal of useful information has been derived from such probe studies. However the important question, ‘Do fluorescent probes perturb biomembranes?’ has not been conclusively answered. Indeed it has been suggested that probes can act as ‘molecular tumours’ and that unless proved otherwise a probe must be suspect of acting as a perturbant of the region it is monitoring [2]. In this communication, we report the use of the fluorescent hydrocarbon pyrene, to monitor structural changes, in lipids caused by ANS binding.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to Elsevier Science Ltd
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Depositing User: M.K Anitha
Date Deposited: 03 Sep 2004
Last Modified: 19 Sep 2010 04:14
URI: http://eprints.iisc.ac.in/id/eprint/1122

Actions (login required)

View Item View Item