Surface Ligand Density Switches Glycovesicles between Monomeric and Multimeric Lectin Recognition

Yadav, S and Naresh, K and Jayaraman, N (2021) Surface Ligand Density Switches Glycovesicles between Monomeric and Multimeric Lectin Recognition. In: ChemBioChem, 22 (3). pp. 485-490.

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Abstract

Carbohydrate-protein interactions define a multitude of cellular recognition events. We present herein synthetic glycovesicles as cell-surface mimics in order to switch the nature of lectin recognition. The covalent glycovesicles, constituted with diacetylene monomers of various ligand densities at their surfaces, are prepared through photo-polymerization. Vesicles with sparsely imbedded ligands engage in a lectin interaction leading to the formation of a dense, crosslinked multimeric complex. On the other hand, vesicles with many ligands, or completely covered with them, switch the lectin interaction to form a fully soluble monomeric complex, without crosslinking. Nanomolar dissociation constants govern these interactions, as assessed by a ligand-displacement assay. The study demonstrates the switching nature – between monomeric and multimeric – of the interaction as a function of ligand density in the vesicles; the results are directly relevant to understanding such a phenomenon occurring at cell surfaces. © 2020 Wiley-VCH GmbH

Item Type:	Journal Article
Publication:	ChemBioChem
Publisher:	Wiley-VCH Verlag
Additional Information:	The copyright for this article belongs to Wiley-VCH Verlag.
Keywords:	carbohydrate; glycolipid; lectin; monomer; polymer; glycoside; lectin; ligand, Article; binding affinity; binding site; cell surface; circular dichroism; complex formation; controlled study; cross linking; dissociation constant; glycovesicle; hydrodynamics; membrane vesicle; photon correlation spectroscopy; polymerization; protein interaction; spectrofluorometry; surface plasmon resonance; synthesis; B lymphocyte; chemical structure; chemistry; human; surface property, B-Lymphocytes; Glycosides; Humans; Lectins; Ligands; Molecular Structure; Surface Properties
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	08 Mar 2023 13:01
Last Modified:	08 Mar 2023 13:01
URI:	https://eprints.iisc.ac.in/id/eprint/80868

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