Sarkar, L and Oko, L and Gupta, S and Bubak, AN and Das, B and Gupta, P and Safiriyu, AA and Singhal, C and Neogi, U and Bloom, D and Banerjee, A and Mahalingam, R and Cohrs, RJ and Koval, M and Shindler, KS and Pal, D and Nagel, M and Sarma, JD (2022) Azadirachta indica A. Juss bark extract and its Nimbin isomers restrict β-coronaviral infection and replication. In: Virology, 569 . pp. 13-28.
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Abstract
Emerging mutations in the SARS-CoV-2 genome pose a challenge for vaccine development and antiviral therapy. The antiviral efficacy of Azadirachta indica bark extract (NBE) was assessed against SARS-CoV-2 and m-CoV-RSA59 infection. Effects of in vivo intranasal or oral NBE administration on viral load, inflammatory response, and histopathological changes were assessed in m-CoV-RSA59-infection. NBE administered inhibits SARS-CoV-2 and m-CoV-RSA59 infection and replication in vitro, reducing Envelope and Nucleocapsid gene expression. NBE ameliorates neuroinflammation and hepatitis in vivo by restricting viral replication and spread. Isolated fractions of NBE enriched in Nimbin isomers shows potent inhibition of m-CoV-RSA59 infection in vitro. In silico studies revealed that NBE could target Spike and RdRp of m-CoV and SARS-CoV-2 with high affinity. NBE has a triterpenoids origin that may allow them to competitively target panoply of viral proteins to inhibit mouse and different strains of human coronavirus infections, suggesting its potential as an antiviral against pan-β-Coronaviruses. © 2022 Elsevier Inc.
| Item Type: | Journal Article |
|---|---|
| Publication: | Virology |
| Publisher: | Elsevier Ltd |
| Additional Information: | The copyright for this article belongs to the authors |
| Department/Centre: | Division of Interdisciplinary Sciences > Computational and Data Sciences |
| Date Deposited: | 11 May 2022 16:28 |
| Last Modified: | 11 May 2022 16:28 |
| URI: | https://eprints.iisc.ac.in/id/eprint/71619 |
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