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Differential expression and antibacterial activity of WFDC10A in the monkey epididymis

Shayu, D and Rao, AJ and Chennakesava, CS (2006) Differential expression and antibacterial activity of WFDC10A in the monkey epididymis. In: Molecular and Cellular Endocrinology, 259 (1-2). pp. 50-56.

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The ability of the epididymis to perform its diverse functions stems from its regionalized gene and protein expression patterns. The differences in the gene expression patterns of the caput and cauda regions of the bonnet monkey epididymis were compared using the technique of differential display reverse transcriptase polymerase chain reaction. A transcript showing homology to human whey acidic protein 10 (hWFDC10A) was highly expressed in the monkey caput region. A peptide P2 was designed spanning a region of the monkey WFDC10A (mWFDC10A), which could inhibit the growth of gram-negative bacterial strains of Escherichia coli. P2 could permeabilize the bacterial cell membrane but was unable to permeabilize mammalian cells as evidenced by the lack of hemolysis upon incubation with the peptide. Expression of genes such as mWFDC10A may be essential in providing the first line of defense against microbial infections to the epididymal tract and thus rendering protection to the male gametes sheltered within the epididymis.

Item Type: Journal Article
Publication: Molecular and Cellular Endocrinology
Publisher: Elsevier
Additional Information: Copyright of this article belongs to Elsevier.
Keywords: Caput;Differential display;Hemolysis;Fluorescence;Innate immunity
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 01 Dec 2006
Last Modified: 19 Sep 2010 04:32
URI: http://eprints.iisc.ac.in/id/eprint/8976

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