Sensory modulation of neuropeptide signaling by CASY-1 gates cholinergic transmission at Caenorhabditis elegans neuromuscular junction

Shahi, N and Thapliyal, S and Babu, K (2025) Sensory modulation of neuropeptide signaling by CASY-1 gates cholinergic transmission at Caenorhabditis elegans neuromuscular junction. In: Journal of Biosciences, 50 (1).

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Abstract

The neuromuscular junction (NMJ) is crucial for understanding the fundamentals of synaptic transmission and activity. Various modulators operate within neuronal circuits, from sensory to motor neurons, to influence synaptic transmission at the NMJ. This study sheds light on the regulation of sensory-evoked cholinergic neurotransmission at motor neurons orchestrated by CASY-1, the mammalian calsyntenin orthologue. We report that the increased excitation�inhibition (E-I) ratio at the NMJ in casy-1 mutants is likely due to its interactions with neuromodulators in sensory neurons. We explored the intricate genetic interactions of CASY-1 with the neuropeptide FLP-21 and its receptor, NPR-1, both of which display simultaneous alterations in cholinergic signaling at the NMJ. Through genetic, pharmacological, and bioimaging-based experiments, we proposed a mechanism by which CASY-1 potentially interacts with the neuropeptide-carrying vesicles to regulate synaptic transmission. The nematode Caenorhabditis elegans serves as an ideal model system for this study, enabling detailed insights into neuromodulatory mechanisms in the neuronal circuit. © Indian Academy of Sciences 2024.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Springer
Additional Information:	The copyright for this article belongs to the Publishers.
Keywords:	aldicarb; CASY 1 protein; FLP 21 peptide; membrane protein; neuropeptide; neuropeptide receptor; neuropeptide receptor 1; unclassified drug, animal cell; Article; assay; Caenorhabditis elegans; cholinergic transmission; coelomocyte uptake assay; controlled study; gene interaction; gene overexpression; motoneuron; mutant; neuromodulation; neuromuscular junction; neuropeptide signaling; nonhuman; protein protein interaction; real time polymerase chain reaction; sensory evoked potential; sensory nerve cell; signal transduction
Department/Centre:	Division of Biological Sciences > Centre for Neuroscience
Date Deposited:	28 Jan 2025 11:11
Last Modified:	28 Jan 2025 11:11
URI:	http://eprints.iisc.ac.in/id/eprint/87300

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