Sugar-Derived Carbon Dots with Variable Fluorescence Blinking Property as a Super-Resolution Imaging Probe

Mukherjee, B and Pant, N and Dolai, J and Joshi, P and Roy, D and Maity, A and Jana, NR (2024) Sugar-Derived Carbon Dots with Variable Fluorescence Blinking Property as a Super-Resolution Imaging Probe. In: Journal of Physical Chemistry C, 128 (45). pp. 19260-19269.

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Abstract

Single-molecule localization microscopy (SMLM) is a powerful technique for breaking the diffraction limit of a light microscope and visualizing the structural details on a nanometer length scale. However, SMLM requires a probe with fluorescence blinking property, and development of a photostable probe with a tunable blinking cycle, high photon count, and low duty cycle is critical. Herein, we report a series of carbon dots (CDs) with variable fluorescence blinking properties that are derived from carbonization of different sugar molecules. We found that the fluorescence blinking property of reducing-sugar-derived CDs differs from that of nonreducing-sugar-derived CDs due to different surface functionalities and structural defects. These CDs have been used for SMLM-based single-particle imaging with a localization precision of 12-28 nm and a resolution of 54-62 nm, and more defective CDs offer better localization precision. The glucose-derived CDs are used for SMLM-based imaging of lysozyme fibrils with 61 nm resolution. This result suggests that CDs with varied fluorescence blinking parameters can be derived for super-resolution imaging applications. © 2024 American Chemical Society.

Item Type:	Journal Article
Publication:	Journal of Physical Chemistry C
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to Journal of Physical Chemistry C
Keywords:	Carbonization; Fluorescence imaging, Breakings; Carbon dots; Derived carbons; Diffraction limits; Fluorescence blinking; Imaging probe; Localisation; Property; Single-molecule localizations; Super resolution imaging, Probes
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	12 Dec 2024 18:57
Last Modified:	12 Dec 2024 18:57
URI:	http://eprints.iisc.ac.in/id/eprint/87025

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