Sasmal, R and Som, A and Kumari, P and Nair, RV and Show, S and Barge, NS and Pahwa, M and Das Saha, N and Rao, S and Vasu, S and Agarwal, R and Agasti, SS (2024) Supramolecular Guest Exchange in Cucurbit7uril for Bioorthogonal Fluorogenic Imaging across the Visible Spectrum. In: ACS Central Science .
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Abstract
Fluorogenic probes that unmask fluorescence signals in response to bioorthogonal reactions are a powerful new addition to biological imaging. They can significantly reduce background fluorescence and minimize nonspecific signals, potentially enabling real-time, high-contrast imaging without the need to wash out excess fluorophores. While diverse classes of highly refined synthetic fluorophores are now readily available, integrating them into a bioorthogonal fluorogenic scheme still requires extensive design efforts and customized structural alterations to optimize quenching mechanisms for each specific fluorophore scaffold. Herein, we present a highly generalizable strategy that can produce an efficient bioorthogonal fluorogenic response from essentially any readily available fluorophore without further structural alterations. We designed this strategy based on the macrocyclic cucurbit7uril (CB7) host, where a fluorogenic response is achieved by programming a guest exchange reaction within the macrocyclic cavity. We employed this strategy to rapidly create fluorogenic probes across the visible spectrum from diverse fluorophore scaffolds, which enabled no-wash imaging in live cells and tissues with minimal background signal. Finally, we demonstrated that this strategy can be combined with metabolic labeling for fluorogenic detection of metabolically tagged mycobacteria under no-wash conditions and paired with covalently clickable probes for high-contrast super-resolution and multiplexed imaging in cells and tissues. © 2024 The Authors. Published by American Chemical Society.
| Item Type: | Journal Article |
|---|---|
| Publication: | ACS Central Science |
| Publisher: | American Chemical Society |
| Additional Information: | The copyright for this article belongs to Authors. |
| Department/Centre: | Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering |
| Date Deposited: | 09 Nov 2024 18:09 |
| Last Modified: | 09 Nov 2024 18:09 |
| URI: | http://eprints.iisc.ac.in/id/eprint/86679 |
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