Functional Conservation of the DDP1-type Inositol Pyrophosphate Phosphohydrolases in Land Plant

Chalak, K and Yadav, R and Liu, G and Rana, P and Jessen, HJ and Laha, D (2024) Functional Conservation of the DDP1-type Inositol Pyrophosphate Phosphohydrolases in Land Plant. In: Biochemistry .

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Abstract

Inositol pyrophosphates (PP-InsPs) are eukaryote-specific second messengers that regulate diverse cellular processes, including immunity, nutrient sensing, and hormone signaling pathways in plants. These energy-rich messengers exhibit high sensitivity to the cellular phosphate status, suggesting that the synthesis and degradation of PP-InsPs are tightly controlled within the cells. Notably, the molecular basis of PP-InsP hydrolysis in plants remains largely unexplored. In this study, we report the functional characterization of MpDDP1, a diadenosine and diphosphoinositol polyphosphate phosphohydrolase encoded by the genome of the liverwort, Marchantia polymorpha. We show that MpDDP1 functions as a PP-InsP phosphohydrolase in different heterologous organisms. Consistent with this finding, M. polymorpha plants defective in MpDDP1 exhibit elevated levels of 1/3-InsP7 and 1/3,5-InsP8, highlighting the contribution of MpDDP1 in regulating PP-InsP homeostasis in planta. Furthermore, our study reveals that MpDDP1 controls thallus development and vegetative reproduction in M. polymorpha. Collectively, this study provides insights into the regulation of specific PP-InsP messengers by DDP1-type phosphohydrolases in land plants. © 2024 American Chemical Society.

Item Type:	Journal Article
Publication:	Biochemistry
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to publisher.
Keywords:	Cellular process; Energy; Functionals; High sensitivity; Hormone signaling; Inositol pyrophosphate; Land plants; Phosphohydrolases; Second messenger; Signalling pathways, Plant diseases
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	09 Nov 2024 18:03
Last Modified:	09 Nov 2024 18:03
URI:	http://eprints.iisc.ac.in/id/eprint/86675

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