Rapid paper-based optical sensing of Spilosoma obliqua nucleopolyhedrovirus via ester hydrolysis

Biswakarma, D and Dey, N and Sivakumar, G and Bhagat, D and Bhattacharya, S (2024) Rapid paper-based optical sensing of Spilosoma obliqua nucleopolyhedrovirus via ester hydrolysis. In: Organic and Biomolecular Chemistry .

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Abstract

We have developed an easily scalable chromogenic probe for the dual-mode sensing of Spilosoma obliqua Nuclear polyhedrosis viruses (SpobNPV) in aqueous media. The mechanistic investigations establish that the imidazole-mediated hydrolysis of acyl ester linkage in which water (general base) acts as a nucleophile induces a pronounced change in the emission colour from blue to cyan. To the best of our knowledge, this is the first attempt at quantifying OBs of SpobNPV using a small molecule-based optical probe with a detection limit of 2.305 � 103 OBs mL�1. The rate of ester hydrolysis was dependent on both substrate and OBs concentration. Due to the naked eye response, paper strips were also developed for the rapid and onsite detection of SpobNPV. The operation procedure is straightforward and does not involve additional sample preparation steps. This makes the present protocol suitable for daily use. Interestingly, the present protocol is also quite efficient in estimating SpobNPV, even in several agricultural crop samples (for at least 15 crops). Such findings will add a new dimension to better managing Spilosoma obliqua and minimizing the extent of crop loss. © 2024 The Royal Society of Chemistry

Item Type:	Journal Article
Publication:	Organic and Biomolecular Chemistry
Publisher:	Royal Society of Chemistry
Additional Information:	The copyright for this article belongs to publisher.
Keywords:	Crops; Esters; Probes; Viruses, Acyl esters; Aqueous media; Chromogenic probes; Dual modes; Ester hydrolysis; Ester linkages; Imidazol; Mechanistics; Nuclear polyhedrosis virus; Optical sensing, Hydrolysis
Department/Centre:	Division of Chemical Sciences > Organic Chemistry Division of Chemical Sciences > Solid State & Structural Chemistry Unit
Date Deposited:	10 Oct 2024 11:45
Last Modified:	10 Oct 2024 11:45
URI:	http://eprints.iisc.ac.in/id/eprint/86400

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