Singh, R and Kansara, K and Yadav, P and Mandal, S and Varshney, R and Gupta, S and Kumar, A and Maiti, PK and Bhatia, D (2024) DNA tetrahedral nanocages as a promising nanocarrier for dopamine delivery in neurological disorders. In: Nanoscale, 16 (32). pp. 15158-15169.
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Abstract
Dopamine is a neurotransmitter in the central nervous system that is essential for many bodily and mental processes, and a lack of it can cause Parkinson's disease. DNA tetrahedral (TD) nanocages are promising in bio-nanotechnology, especially as a nanocarrier. TD is highly programmable, biocompatible, and capable of cell differentiation and proliferation. It also has tissue and blood-brain barrier permeability, making it a powerful tool that could overcome potential barriers in treating neurological disorders. In this study, we used DNA TD as a carrier for dopamine to cells and zebrafish embryos. We investigated the mechanism of complexation between TD and dopamine hydrochloride using gel electrophoresis, fluorescence and circular dichroism (CD) spectroscopy, atomic force microscopy (AFM), and molecular dynamic (MD) simulation tools. Further, we demonstrate that these dopamine-loaded DNA TD nanostructures enhanced cellular uptake and differentiation ability in SH-SY5Y neuroblastoma cells. Furthermore, we extended the study to zebrafish embryos as a model organism to examine survival and uptake. The research provides valuable insights into the complexation mechanism and cellular uptake of dopamine-loaded DNA tetrahedral nanostructures, paving the way for further advancements in nanomedicine for Parkinson's disease and other neurological disorders. © 2024 The Royal Society of Chemistry.
| Item Type: | Journal Article |
|---|---|
| Publication: | Nanoscale |
| Publisher: | Royal Society of Chemistry |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | Amines; Biocompatibility; Circular dichroism spectroscopy; Dichroism; Electrophoresis; Medical nanotechnology; Molecular dynamics; Neurodegenerative diseases; Neurophysiology, Bio-nanotechnologies; Cellular uptake; Central nervous systems; Dopamine; Mental process; Nanocages; Nanocarriers; Neurological disorders; Parkinson's disease; Zebrafish embryos, DNA |
| Department/Centre: | Division of Physical & Mathematical Sciences > Physics |
| Date Deposited: | 30 Aug 2024 06:02 |
| Last Modified: | 30 Aug 2024 06:02 |
| URI: | http://eprints.iisc.ac.in/id/eprint/86052 |
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