Dhanjal, DS and Singh, S and Kumar, V and Ramamurthy, PC and Chopra, C and Wani, AK and Singh, R and Singh, J (2023) Isolation and Characterization of Cellulase-Producing Myxobacterial Strain from the Unique Niche of Mirgund Wetland from the North-Western Himalayas. In: Journal of Applied Biology and Biotechnology, 11 (5). pp. 119-125.
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Abstract
Microbes are stated to be an excellent source of novel enzymes and secondary metabolites having potential industrial applications. Many attempts have been made around the globe to unravel novel bioactive molecules from microbes inhabiting different unexplored ecological niches. Myxobacteria, a member of δ-proteobacteria belonging to the order Myxococcales, is a gram-negative rod-shaped bacterium well-known for coordinated movement, fruiting body formation during starvation, gliding mobility, and a well-developed communication system. They have emerged as valuable producers of various natural products belonging to diverse chemical classes with wide-spectrum and unique bioactive molecules that have attracted academicians and researchers for their exploration. The bioactive molecules obtained from myxobacteria have been proclaimed to have the ability to break down macromolecules of biological origin by secreting a large number of extra-cellular enzymes. The current study reports the isolation of myxobacteria from the Mirgund Wetland in the North-Western Himalayas through the dung-baiting method. The isolated myxobacteria showed characteristic morphological features of myxobacteria, like the formation of fruiting bodies and swarming patterns on the solid Casitone-enriched media. 16S ribosomal RNA gene sequencing of the strain showed 100 similarity with Myxococcus spp. The isolated strain produced an active cellulase with an optimum activity at 40°C and pH 7.0 using the 3,5-dinitro salicylic acid method when cultured on minimal broth containing carboxymethyl cellulose. This is the first report of a myxobacterial strain and enzyme from the Mirgund Wetland of the Union Territory of Jammu and Kashmir and highlights the immense potential of the hidden wealth of enzymes in the area. © 2023 Daljeet Singh Dhanjal, et al.
| Item Type: | Journal Article |
|---|---|
| Publication: | Journal of Applied Biology and Biotechnology |
| Publisher: | Open Science Publishers LLP Inc. |
| Additional Information: | The copyright for this article belongs to authors. |
| Keywords: | cellulase; genomic DNA; RNA 16S, Article; bacterial strain; bacterium identification; bacterium isolation; DNA isolation; enzyme activity; enzyme localization; gene amplification; gene sequence; genetic engineering; moisture; myxobacterial strain; Myxococcus fulvus; Myxococcus xanthus; nonhuman; pH; phylogenetic tree; physical chemistry; polymerase chain reaction |
| Department/Centre: | Division of Interdisciplinary Sciences > Interdisciplinary Centre for Water Research |
| Date Deposited: | 14 Dec 2024 00:53 |
| Last Modified: | 14 Dec 2024 00:53 |
| URI: | http://eprints.iisc.ac.in/id/eprint/85498 |
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