Advancing Diagnostics: Evaluating the Potential of Nanoparticle-Based Immunoassays as PCR Alternatives for Disease Confirmation

Kurdekar, A and Kamisetti, V (2024) Advancing Diagnostics: Evaluating the Potential of Nanoparticle-Based Immunoassays as PCR Alternatives for Disease Confirmation. In: Current Analytical Chemistry, 20 (5). pp. 287-294.

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Abstract

In diagnostic technologies, search for quick, accurate, and cost-efficient ways to confirm diseases has prompted the investigation of alternative methodologies to classic PCR-based tests. This article delves into the growing field of nanoparticle-based immunoassays, offering a comprehensive evaluation of their potential as viable alternatives to PCR for disease diagnostics. We discuss the basic principles of nanoparticle-based immunoassays, highlighting their distinctive ability to combine the specialized binding characteristics of antibodies with the improved detection capacities of nanoparticles. The article compares the performance attributes of nanoparticle-based immunoassays with PCR, focusing on sensitivity, specificity, and detection speed. We further delve into a novel diagnostic technology, immuno-PCR, which integrates the strengths of immunoassays and PCR techniques. Healthcare systems and stakeholders must comprehend and implement novel diagnostic procedures emphasizing accuracy and sensitivity as the diagnostic landscape advances globally. This review contributes to the advancement of diagnostics by synthesizing current research and suggesting future directions for development. It highlights the transformative potential of current and emerging methods in shaping the future of disease diagnostics. © 2024 Bentham Science Publishers.

Item Type:	Editorials/Short Communications
Publication:	Current Analytical Chemistry
Publisher:	Bentham Science Publishers
Additional Information:	The copyright for this article belongs to Bentham Science Publishers.
Keywords:	Chemical detection; Diagnosis; Polymerase chain reaction, 'current; Basic principles; Comprehensive evaluation; Cost-efficient; Diagnostic technologies; Disease diagnostics; Immunoassay; Nanoparticle-based immunoassays; Novel diagnostics; PCR, Nanoparticles
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	22 Aug 2024 05:44
Last Modified:	22 Aug 2024 05:44
URI:	http://eprints.iisc.ac.in/id/eprint/85485

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