More, DA and Singh, N and Mishra, R and Muralidharan, HP and Gopinath, KS and Gopal, C and Kumar, A (2024) Intronic miR-6741-3p targets the oncogene SRSF3: Implications for oral squamous cell carcinoma pathogenesis. In: PLoS ONE, 19 (5 May).
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Abstract
Epigenetic silencing through methylation is one of the major mechanisms for downregulation of tumor suppressor miRNAs in various malignancies. The aim of this study was to identify novel tumor suppressor miRNAs which are silenced by DNA hypermethylation and investigate the role of at least one of these in oral squamous cell carcinoma (OSCC) pathogenesis. We treated cells from an OSCC cell line SCC131 with 5-Azacytidine, a DNA methyltransferase inhibitor, to reactivate tumor suppressor miRNA genes silenced/downregulated due to DNA methylation. At 5-day post-treatment, total RNA was isolated from the 5-Azacytidine and vehicle control-treated cells. The expression of 2,459 mature miRNAs was analysed between 5-Azacytidine and control-treated OSCC cells by the microRNA microarray analysis. Of the 50 miRNAs which were found to be upregulated following 5-Aza-cytidine treatment, we decided to work with miR-6741-3p in details for further analysis, as it showed a mean fold expression of >4.0. The results of qRT-PCR, Western blotting, and dual-luciferase reporter assay indicated that miR-6741-3p directly targets the oncogene SRSF3 at the translational level only. The tumor-suppressive role of miR-6741-3p was established by various in vitro assays and in vivo study in NU/J athymic nude mice. Our results revealed that miR-6741-3p plays a tumor-suppressive role in OSCC pathogenesis, in part, by directly regulating SRSF3. Based on our observations, we propose that miR-6741-3p may serve as a potential biological target in tumor diagnostics, prognostic evaluation, and treatment of OSCC and perhaps other malignancies. © 2024 More et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
| Item Type: | Journal Article |
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| Publication: | PLoS ONE |
| Publisher: | Public Library of Science |
| Additional Information: | The copyright for this article belongs t Authors. |
| Keywords: | azacitidine; microRNA; azacitidine; serine arginine rich splicing factor; SRSF3 protein, human, Akt/mTOR signaling; apoptosis; Article; carcinogenesis; cell proliferation; down regulation; epigenetics; gene expression; gene overexpression; genetic transfection; human; luciferase assay; MAPK signaling; methylation; microarray analysis; mouth squamous cell carcinoma; pathogenesis; Pi3K/Akt signaling; prognosis; real time polymerase chain reaction; SRSF3 gne; tumor diagnosis; tumor growth; tumor suppressor gene; tumor volume; tumor xenograft; upregulation; Western blotting; animal; DNA methylation; drug effect; gene expression regulation; genetics; intron; metabolism; mouse; mouth tumor; nude mouse; oncogene; pathology; squamous cell carcinoma; tumor cell line, Animals; Azacitidine; Carcinoma, Squamous Cell; Cell Line, Tumor; DNA Methylation; Gene Expression Regulation, Neoplastic; Humans; Introns; Mice; Mice, Nude; MicroRNAs; Mouth Neoplasms; Oncogenes; Serine-Arginine Splicing Factors |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
| Date Deposited: | 07 Aug 2024 06:28 |
| Last Modified: | 07 Aug 2024 06:28 |
| URI: | http://eprints.iisc.ac.in/id/eprint/85255 |
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