Global transcriptome analysis reveals Salmonella Typhimurium employs nitrate metabolism to combat bile stress

Singh, M and Chandra, D and Jagdish, S and Nandi, D (2024) Global transcriptome analysis reveals Salmonella Typhimurium employs nitrate metabolism to combat bile stress. In: FEBS Letters, 598 . pp. 1605-1619.

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Abstract

Salmonella Typhimurium is an enteric pathogen that is highly tolerant to bile. Next-generation mRNA sequencing was performed to analyze the adaptive responses to bile in two S. Typhimurium strains: wild type (WT) and a mutant lacking cold shock protein E (�cspE). CspE is an RNA chaperone which is crucial for survival of S. Typhimurium during bile stress. This study identifies transcriptional responses in bile-tolerant WT and bile-sensitive �cspE. Upregulation of several genes involved in nitrate metabolism was observed, including fnr, a global regulator of nitrate metabolism. Notably, �fnr was susceptible to bile stress. Also, complementation with fnr lowered reactive oxygen species and enhanced the survival of bile-sensitive �cspE. Importantly, intracellular nitrite amounts were highly induced in bile-treated WT compared to �cspE. Also, the WT strain pre-treated with nitrate displayed better growth with bile. These results demonstrate that nitrate-dependent metabolism promotes adaptation of S. Typhimurium to bile. © 2024 Federation of European Biochemical Societies.

Item Type:	Journal Article
Publication:	FEBS Letters
Publisher:	John Wiley and Sons Inc
Additional Information:	The copyright for this article belongs to John Wiley and Sons Inc.
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	30 Aug 2024 12:13
Last Modified:	30 Aug 2024 12:13
URI:	http://eprints.iisc.ac.in/id/eprint/84926

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