Role of Rmd9p in 3�-end processing of mitochondrial 15S rRNA in Saccharomyces cerevisiae

Singh, J and Singh, S and Emam, EAF and Varshney, U (2024) Role of Rmd9p in 3�-end processing of mitochondrial 15S rRNA in Saccharomyces cerevisiae. In: Mitochondrion, 76 .

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Abstract

Ribosome biogenesis, involving processing/assembly of rRNAs and r-proteins is a vital process. In Saccharomyces cerevisiae mitochondria, ribosomal small subunit comprises 15S rRNA (15S). While the 15S 5�-end processing uses Ccm1p and Pet127p, the mechanisms of the 3�-end processing remain unclear. We reveal involvement of Rmd9p in safeguarding/processing 15S 3�-end. Rmd9p deficiency results in a cleavage at a position 183 nucleotides upstream of 15S 3�-end, and in the loss of the 3�-minor domain. Rmd9p binds to the sequences in the 3'-end region of 15S, and a genetic interaction between rmd9 and dss1 indicates that Rmd9p regulates/limits mtEXO activity during the 3�-end spacer processing. © 2024 Elsevier B.V. and Mitochondria Research Society

Item Type:	Journal Article
Publication:	Mitochondrion
Publisher:	Elsevier B.V.
Additional Information:	The copyright for this article belongs to Elsevier B.V.
Keywords:	mitochondrial 15S rRNA; ribosome RNA; unclassified drug, Article; binding affinity; controlled study; dss1 gene; gene; gene control; gene function; gene interaction; gene loss; mitochondrial biogenesis; nonhuman; rmd9 gene; RNA 3' end processing; RNA analysis; RNA sequence; RNA translation; Saccharomyces cerevisiae
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	10 Jul 2024 06:02
Last Modified:	10 Jul 2024 06:02
URI:	http://eprints.iisc.ac.in/id/eprint/84769

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