Dubey, H and Pradeep, AR and Neog, K and Debnath, R and Aneesha, PJ and Shah, SK and Kamatchi, I and Ponnuvel, KM and Ramesha, A and Vijayan, K and Nongthomba, U and Bora, U and Vankadara, S and VijayaKumari, KM and Arunkumar, KP (2024) Genome sequencing and assembly of Indian golden silkmoth, Antheraea assamensis Helfer (Saturniidae, Lepidoptera). In: Genomics, 116 (3).
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Abstract
Muga silkworm (Antheraea assamensis), one of the economically important wild silkmoths, is unique among saturniid silkmoths. It is confined to the North-eastern part of India. Muga silk has the highest value among the other silks. Unlike other silkmoths, A. assamensis has a low chromosome number (n = 15), and ZZ/ZO sex chromosome system. Here, we report the first high-quality draft genome of A. assamensis, assembled by employing the Illumina and PacBio sequencing platforms. The assembled genome of A. assamensis is 501.18 Mb long, with 2697 scaffolds and an N50 of 683.23 Kb. The genome encompasses 18,385 protein-coding genes, 86.29 of which were functionally annotated. Phylogenetic analysis of A. assamensis revealed its divergence from other Antheraea species approximately 28.7 million years ago. Moreover, an investigation into detoxification-related gene families, CYP450, GST, and ABC-transporter, revealed a significant expansion in A. assamensis as compared to the Bombyx mori. This expansion is comparable to Spodoptera litura, suggesting adaptive responses linked to the polyphagous behavior observed in these insects. This study provides valuable insights into the molecular basis of evolutionary divergence and adaptations in muga silkmoth. The genome assembly reported in this study will significantly help in the functional genomics studies on A. assamensis and other Antheraea species along with comparative genomics analyses of Bombycoidea insects. © 2024
| Item Type: | Journal Article |
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| Publication: | Genomics |
| Publisher: | Academic Press Inc. |
| Additional Information: | The copyright for this article belongs to authors. |
| Keywords: | ABC transporter; cytochrome P450; genomic DNA; glutathione transferase, adaptation; Antheraea assamensis; Article; Bombycoidea; Bombyx mori; chromosome number; comparative genomics; controlled study; detoxification; evolution; functional genomics; gene sequence; genetic parameters; genetic variability; genome assembly; genome size; high throughput sequencing; hyperphagia; illumina sequencing; insect genetics; male; molecular phylogeny; multigene family; nonhuman; PacBio sequencing; species comparison; Spodoptera litura |
| Department/Centre: | Others |
| Date Deposited: | 10 Jul 2024 05:59 |
| Last Modified: | 10 Jul 2024 05:59 |
| URI: | http://eprints.iisc.ac.in/id/eprint/84768 |
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