Three-Dimensional Extrusion Printed Urinary Specific Grafts: Mechanistic Insights into Buildability and Biophysical Properties

Chowdhury, SR and Mondal, G and Ratnayake, P and Basu, B (2024) Three-Dimensional Extrusion Printed Urinary Specific Grafts: Mechanistic Insights into Buildability and Biophysical Properties. In: ACS Biomaterials Science and Engineering, 10 (2). pp. 1040-1061.

PDF acs_bio_sci_eng_10_2_2024.pdf - Published Version Restricted to Registered users only Download (4MB)

Abstract

The compositional formulations and the optimization of process parameters to fabricate hydrogel scaffolds with urological tissue-mimicking biophysical properties are not yet extensively explored, including a comprehensive assessment of a spectrum of properties, such as mechanical strength, viscoelasticity, antimicrobial property, and cytocompatibility. While addressing this aspect, the present work provides mechanistic insights into process science, to produce shape-fidelity compliant alginate-based biomaterial ink blended with gelatin and synthetic nanocellulose. The composition-dependent pseudoplasticity, viscoelasticity, thixotropy, and gel stability over a longer duration in physiological context have been rationalized in terms of intermolecular hydrogen bonding interactions among the biomaterial ink constituents. By varying the hybrid hydrogel ink composition within a narrow compositional window, the resulting hydrogel closely mimics the natural urological tissue-like properties, including tensile stretchability, compressive strength, and biophysical properties. Based on the printability assessment using a critical analysis of gel strength, we have established the buildability of the acellular hydrogel ink and have been successful in fabricating shape-fidelity compliant urological patches or hollow cylindrical grafts using 3D extrusion printing. Importantly, the new hydrogel formulations with good hydrophilicity, support fibroblast cell proliferation and inhibit the growth of Gram-negative E. coli bacteria. These attributes were rationalized in terms of nanocellulose-induced physicochemical changes on the scaffold surface. Taken together, the present study uncovers the process-science-based understanding of the 3D extrudability of the newly formulated alginate-gelatin-nanocellulose-based hydrogels with urological tissue-specific biophysical, cytocompatibility, and antibacterial properties. © 2024 American Chemical Society.

Item Type:	Journal Article
Publication:	ACS Biomaterials Science and Engineering
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to the American Chemical Society.
Keywords:	Biomechanics; Cell culture; Cell proliferation; Compressive strength; Escherichia coli; Extrusion; Hydrogels; Hydrogen; Hydrogen bonds; Nanocellulose; Scaffolds (biology); Tensile strength; Tissue; Viscoelasticity, 3-D printing; 3D-printing; Biophysical properties; Intermolecular hydrogen bonding; Mechanistics; Nano-cellulose; Property; Rheological property; Tissue specifics; Tissue-specific graft, 3D printing, alginic acid; biomaterial; gelatin; hydrogel; nanocellulose, antibacterial activity; antimicrobial activity; article; cell proliferation; compressive strength; controlled study; fibroblast; hydrogen bond; hydrophilicity; male; nonhuman; pharmaceutics; physical chemistry; thixotropy; three dimensional printing; urine; viscoelasticity
Department/Centre:	Division of Chemical Sciences > Materials Research Centre
Date Deposited:	09 Apr 2024 10:50
Last Modified:	09 Apr 2024 10:50
URI:	https://eprints.iisc.ac.in/id/eprint/84688

Actions (login required)

View Item