Heterologous expression, biochemical characterization and prospects for insecticide biosensing potential of carboxylesterase Ha006a from Helicoverpa armigera

Kaur, H and Rode, S and Lonare, S and Demiwal, P and Narasimhappa, P and Arun, E and Kumar, R and Das, J and Ramamurthy, PC and Sircar, D and Sharma, AK (2024) Heterologous expression, biochemical characterization and prospects for insecticide biosensing potential of carboxylesterase Ha006a from Helicoverpa armigera. In: Pesticide Biochemistry and Physiology, 200 .

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Abstract

Enzymes have attracted considerable scientific attention for their crucial role in detoxifying a wide range of harmful compounds. In today's global context, the extensive use of insecticides has emerged as a significant threat to the environment, sparking substantial concern. Insects, including economically important pests like Helicoverpa armigera, have developed resistance to conventional pest control methods through enzymes like carboxyl/cholinesterases. This study specifically focuses on a notable carboxyl/cholinesterase enzyme from Helicoverpa armigera (Ha006a), with the goal of harnessing its potential to combat environmental toxins. A total of six insecticides belonging to two different classes displayed varying inhibitory responses towards Ha006a, thereby rendering it effective in detoxifying a broader spectrum of insecticides. The significance of this research lies in discovering the bioremediation property of Ha006a, as it hydrolyzes synthetic pyrethroids (fenvalerate, λ-cyhalothrin and deltamethrin) and sequesters organophosphate (paraoxon ethyl, profenofos, and chlorpyrifos) insecticides. Additionally, the interaction studies between organophosphate insecticides and Ha006a helped in the fabrication of a novel electroanalytical sensor using a modified carbon paste electrode (MCPE). This sensor boasts impressive sensitivity, with detection limits of 0.019 μM, 0.15 μM, and 0.025 μM for paraoxon ethyl, profenofos, and chlorpyrifos, respectively. This study provides a comprehensive biochemical and biophysical characterization of the purified esterase Ha006a, showcasing its potential to remediate different classes of insecticides. © 2023

Item Type:	Journal Article
Publication:	Pesticide Biochemistry and Physiology
Publisher:	Academic Press Inc.
Additional Information:	The copyright for this article belongs to Academic Press Inc.
Department/Centre:	Division of Interdisciplinary Sciences > Interdisciplinary Centre for Water Research
Date Deposited:	23 Apr 2024 06:32
Last Modified:	23 Apr 2024 06:32
URI:	https://eprints.iisc.ac.in/id/eprint/84647

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