Divergent Roles of Escherichia Coli Encoded Lon Protease in Imparting Resistance to Uncouplers of Oxidative Phosphorylation: Roles of marA, rob, soxS and acrB

Verma, T and Nandini, SS and Singh, V and Raghavan, A and Annappa, H and Bhaskarla, C and Dubey, AK and Nandi, D (2024) Divergent Roles of Escherichia Coli Encoded Lon Protease in Imparting Resistance to Uncouplers of Oxidative Phosphorylation: Roles of marA, rob, soxS and acrB. In: Current Microbiology, 81 (4).

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Abstract

Uncouplers of oxidative phosphorylation dissipate the proton gradient, causing lower ATP production. Bacteria encounter several non-classical uncouplers in the environment, leading to stress-induced adaptations. Here, we addressed the molecular mechanisms responsible for the effects of uncouplers in Escherichia coli. The expression and functions of genes involved in phenotypic antibiotic resistance were studied using three compounds: two strong uncouplers, i.e., Carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and 2,4-Dinitrophenol (DNP), and one moderate uncoupler, i.e., Sodium salicylate (NaSal). Quantitative expression studies demonstrated induction of transcripts encoding marA, soxS and acrB with NaSal and DNP, but not CCCP. Since MarA and SoxS are degraded by the Lon protease, we investigated the roles of Lon using a lon-deficient strain (�lon). Compared to the wild-type strain, �lon shows compromised growth upon exposure to NaSal or 2, 4-DNP. This sensitivity is dependent on marA but not rob and soxS. On the other hand, the �lon strain shows enhanced growth in the presence of CCCP, which is dependent on acrB. Interestingly, NaSal and 2,4-DNP, but not CCCP, induce resistance to antibiotics, such as ciprofloxacin and tetracycline. This study addresses the effects of uncouplers and the roles of genes involved during bacterial growth and phenotypic antibiotic resistance. Strong uncouplers are often used to treat wastewater, and these results shed light on the possible mechanisms by which bacteria respond to uncouplers. Also, the rampant usage of some uncouplers to treat wastewater may lead to the development of antibiotic resistance. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024.

Item Type:	Journal Article
Publication:	Current Microbiology
Publisher:	Springer
Additional Information:	The copyright for this article belongs to Springer.
Keywords:	2,4 dinitrophenol; carbonyl cyanide chlorophenylhydrazone; chloramphenicol; ciprofloxacin; endopeptidase La; kanamycin; salicylate sodium; tetracycline; adenosine triphosphate; endopeptidase La; proton, acrB gene; antibiotic resistance; Article; bacterial gene; bacterial growth; bacterial strain; chemical stress; controlled study; Escherichia coli; gene expression level; gene function; marA gene; minimum inhibitory concentration; nonhuman; oxidative phosphorylation; phenotype; quantitative analysis; rob gene; soxS gene; upregulation; waste water management; article; commercial phenomena; pharmacology; physiological stress; wild type, Anti-Bacterial Agents; Carbonyl Cyanide m-Chlorophenyl Hydrazone; Dinitrophenols; Escherichia coli; Escherichia coli Proteins; Oxidative Phosphorylation; Protease La; Wastewater
Department/Centre:	Division of Biological Sciences > Biochemistry Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering UG Programme
Date Deposited:	17 May 2024 05:25
Last Modified:	17 May 2024 05:25
URI:	https://eprints.iisc.ac.in/id/eprint/84630

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