A protein-based self-healing hydrogel for prolonged antimicrobial drug delivery with synergistic activity

Mondal, A and Chaudhury, SI and Lyndem, S and Rana, S and Nongbri, DL and Aguan, K and Singha Roy, A (2024) A protein-based self-healing hydrogel for prolonged antimicrobial drug delivery with synergistic activity. In: New Journal of Chemistry, 48 (11). pp. 4865-4879.

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Abstract

This study focuses on synthesizing an injectable and self-healing hydrogel from an abundant serum protein (bovine serum albumin, BSA) to control antimicrobial drug delivery impeding bacterial infections. Hydrogels were synthesized through a green synthesis route without using any crosslinking agent and characterized for their cross-linking, surface morphology, self-healing, viscoelasticity, injectability, and swelling and degradation properties. The prepared hydrogel has distinctive autofluorescence properties, as observed from the wavelength-dependent steady-state fluorescence spectral measurements. When lomefloxacin and doxycycline were incorporated into the hydrogel network, it allowed the controlled release of both antibiotics over at least 24 hours, with a release rate of over 90. Hydrogels containing lomefloxacin and doxycycline were effectively used against E. coli and E. faecalis bacterial strains; their antibacterial effects were maintained for 24 hours. The live/dead cell test indicated that the hydrogel is effective against both (Gram-positive and Gram-negative) bacteria strains. Furthermore, our studies on bacteria encapsulation, specifically using E. coli, confirmed that the hydrogel is non-toxic, and the hydrogel demonstrates biocompatibility as the bacteria successfully grow within the hydrogel matrix. To assess biocompatibility, we conducted a cell attachment experiment with HEK 293 cells, and the results suggest that this hydrogel could be used for 3D cell culturing in the future. © 2024 The Royal Society of Chemistry.

Item Type:	Journal Article
Publication:	New Journal of Chemistry
Publisher:	Royal Society of Chemistry
Additional Information:	The copyright for this article belongs to Royal Society of Chemistry.
Keywords:	Biocompatibility; Biosynthesis; Cells; Controlled drug delivery; Cytology; Escherichia coli; Mammals; Morphology; Proteins; Self-healing materials; Surface morphology; Targeted drug delivery, Antimicrobial drug; Bovine serum albumins; Doxycycline; E. coli; Injectables; Lomefloxacin; Property; Self-healing; Serum proteins; Synergistic activity, Hydrogels
Department/Centre:	Division of Chemical Sciences > Materials Research Centre
Date Deposited:	15 May 2024 04:54
Last Modified:	15 May 2024 04:54
URI:	https://eprints.iisc.ac.in/id/eprint/84463

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