Con A lectin binding by synthetic bivalent arabinomannan tri- and pentasaccharides reveals connectivity-dependent functional valencies

Parija, I and Yadav, S and Jayaraman, N (2024) Con A lectin binding by synthetic bivalent arabinomannan tri- and pentasaccharides reveals connectivity-dependent functional valencies. In: Carbohydrate Research, 536 .

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Abstract

Lectin Con A, with specificity to interact with α-D-mannopyranoside, achieves tight binding affinity with the aid of optimal multivalent ligand valencies, distances and orientations between the ligands. A series of synthetic arabinomannans, possessing arabinan core and mannan at the non-reducing ends, is studied to assess the above constraints involved with lectin binding in this report. Trisaccharides, with (1 � 2)(1 � 3), (1 � 2)(1 � 5) and (1 � 3)(1 � 5) glycosidic bond connectivities, and a pentasaccharide with mannopyranosides at the non-reducing ends are synthesized. The binding affinities of the mannose bivalent ligands are studied with tetrameric Con A lectin by isothermal titration calorimetry (ITC). Among the derivatives, trisaccharide with (1 � 2)(1 � 3) glycosidic bond connectivity and the pentasaccharide undergo lectin interaction, clearly fulfilling the bivalent structural and functional valencies. Remaining oligosaccharides exhibit only a functional monovalency, defying the bivalent structural valency. The trisaccharide fulfilling the structural and functional valencies represent the smallest bivalent ligand, undergoing the lectin interaction in a trans-mode. © 2024 Elsevier Ltd

Item Type:	Journal Article
Publication:	Carbohydrate Research
Publisher:	Elsevier Ltd
Additional Information:	The copyright for this article belongs to Elsevier Ltd.
Keywords:	Binding energy; Calorimeters; Calorimetry; Isotherms; Proteins; Titration, Binding affinities; Glycosylations; Isothermal titration calorimetry; Lectin; Lectin binding; Mannopyranosides; Multivalency; Pentasaccharides; Reducing ends; Valencies, Ligands, arabinomannan; concanavalin A; glycoside; lectin; ligand; mannan; mannose; oligosaccharide; protein binding; trisaccharide, Concanavalin A; Glycosides; Lectins; Ligands; Mannans; Mannose; Oligosaccharides; Protein Binding; Trisaccharides
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	14 May 2024 05:12
Last Modified:	14 May 2024 05:12
URI:	https://eprints.iisc.ac.in/id/eprint/84425

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