Prolonged exposure to lactate causes TREK1 channel clustering in rat hippocampal astrocytes

Ghatak, S and Kumar Sikdar, S (2024) Prolonged exposure to lactate causes TREK1 channel clustering in rat hippocampal astrocytes. In: Neuroscience Letters, 821 .

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Abstract

Increased concentrations of lactate (15�30 mM) are associated with and found to be neuroprotective in various brain pathophysiology. In our earlier studies we showed that high levels of lactate can increase TREK1 channel activity and expression within 1 h. TREK1 channels are two pore domain leak potassium ion channels that are upregulated during cerebral ischemia, epilepsy and other brain pathologies. They play a prominent neuroprotective role against excitotoxicity. Although it has been previously shown that chronic application of lactate (6 h) causes increased gene transcription and protein expression, we observe clustering of TREK1 channels that is dependent on time of exposure (3�6 h) and concentration of lactate (15�30 mM). Using immunofluorescence techniques and image analysis, we show that the clustering of TREK1 channels is dependent on the actin cytoskeletal network of the astrocytes. Clustering of TREK1 channels can augment astrocytic functions during pathophysiological conditions and have significant implications in lactate mediated neuroprotection. © 2023 Elsevier B.V.

Item Type:	Journal Article
Publication:	Neuroscience Letters
Publisher:	Elsevier Ireland Ltd
Additional Information:	The copyright for this article belongs to Author.
Keywords:	actin; cytochalasin D; glial fibrillary acidic protein; lactic acid; potassium channel; TWIK related potassium channel 1; unclassified drug; lactic acid; tandem pore domain potassium channel, actin filament; animal cell; animal experiment; animal tissue; Article; astrocyte; astrocyte culture; brain ischemia; cell viability; concentration (parameter); controlled study; cytoskeleton; hippocampus; image analysis; immunocytochemistry; immunofluorescence assay; in vitro study; internalization (cell); long term exposure; molecular pathology; neuroprotection; nonhuman; protein expression; rat; Wistar rat; animal; astrocyte; brain ischemia; genetics; hippocampus; metabolism, Animals; Astrocytes; Brain Ischemia; Hippocampus; Lactic Acid; Potassium Channels, Tandem Pore Domain; Rats
Department/Centre:	Division of Physical & Mathematical Sciences > Physics
Date Deposited:	01 Mar 2024 07:24
Last Modified:	01 Mar 2024 07:24
URI:	https://eprints.iisc.ac.in/id/eprint/83926

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