Biochemical characterization of lipid metabolic genes of Aurantiochytrium limacinum

Dalmia, A and Daga, P and Datey, A and Chakravortty, D and Tumaney, AW (2024) Biochemical characterization of lipid metabolic genes of Aurantiochytrium limacinum. In: International Journal of Biological Macromolecules, 259 .

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Abstract

Docosahexaenoic acid (DHA) is known to have numerous health benefits and immense dietary value. There is a pressing need to have a deeper understanding of DHA metabolism. Acyl CoA: Diacylglycerol Acyltransferase (DGAT) is an important enzyme of lipid anabolism and an essential piece of the puzzle. Aurantiochytrium limacinum, a primary producer of DHA, is a good model for studying DHA metabolism. Thus, we aimed to investigate important lipid metabolic genes from A. limacinum. We cloned four putative DGATs (DGAT2a, DGAT2b, DGAT2c, and DGAT2d) from A. limacinum and performed detailed in vivo and in vitro characterization. Functional characterization showed that not all the studied genes exhibited DGAT activity. DGAT2a and DGAT2d conferred DGAT activity whereas DGAT2b showed wax synthase (WS) activity and DGAT2c showed dual function of both WS and DGAT. Based on their identified function, DGAT2b and DGAT2c were renamed as AlWS and AlWS/DGAT respectively. DGAT2a was found to exhibit a preference for DHA as a substrate. DGAT2d was found to have robust activity and emerged as a promising candidate for genetic engineering aimed at increasing oil yield. The study enriches our knowledge of lipid biosynthetic enzymes in A. limacinum, which can be utilized to design suitable application strategies. © 2024 Elsevier B.V.

Item Type:	Journal Article
Publication:	International Journal of Biological Macromolecules
Publisher:	Elsevier B.V.
Additional Information:	The copyright for this article belongs to Elsevier B.V.
Keywords:	Cloning; Enzymes; Genes; Metabolic engineering; Unsaturated fatty acids, Aurantiochytria limacinum; Aurantiochytrium; Biochemical characterization; Diacylglycerol acyltransferases; Docosahexaenoic acid; Health benefits; Metabolic genes; Pressung; Synthases; Wax esters, Metabolism, diacylglycerol acyltransferase 2; diacylglycerol acyltransferase 2a; diacylglycerol acyltransferase 2b; diacylglycerol acyltransferase 2c; diacylglycerol acyltransferase 2d; docosahexaenoic acid; synthetase; unclassified drug; wax synthase, Article; Aurantiochytrium limacinum; controlled study; enzyme activity; enzyme analysis; enzyme substrate; fatty acid metabolism; functional assessment; gene; gene function; genetic engineering; in vitro study; in vivo study; lipid metabolic gene; lipogenesis; marine alga; molecular cloning; nonhuman
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	01 Mar 2024 07:09
Last Modified:	01 Mar 2024 07:09
URI:	https://eprints.iisc.ac.in/id/eprint/83919

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