Photo-Controlled Gating of Selective Bacterial Membrane Interaction and Enhanced Antibacterial Activity for Wound Healing

Sahoo, J and Sahoo, S and Subramaniam, Y and Bhatt, P and Rana, S and De, M (2024) Photo-Controlled Gating of Selective Bacterial Membrane Interaction and Enhanced Antibacterial Activity for Wound Healing. In: Angewandte Chemie - International Edition, 63 (1).

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Abstract

Reversible biointerfaces are essential for on-demand molecular recognition to regulate stimuli-responsive bioactivity such as specific interactions with cell membranes. The reversibility on a single platform allows the smart material to kill pathogens or attach/detach cells. Herein, we introduce a 2D-MoS2 functionalized with cationic azobenzene that interacts selectively with either Gram-positive or Gram-negative bacteria in a light-gated fashion. The trans conformation (trans-Azo-MoS2) selectively kills Gram-negative bacteria, whereas the cis form (cis-Azo-MoS2), under UV light, exhibits antibacterial activity against Gram-positive strains. The mechanistic investigation indicates that the cis-Azo-MoS2 exhibits higher affinity towards the membrane of Gram-positive bacteria compared to trans-Azo-MoS2. In case of Gram-negative bacteria, trans-Azo-MoS2 internalizes more efficiently than cis-Azo-MoS2 and generates intracellular ROS to kill the bacteria. While the trans-Azo-MoS2 exhibits strong electrostatic interactions and internalizes faster into Gram-negative bacterial cells, cis-Azo-MoS2 primarily interacts with Gram-positive bacteria through hydrophobic and H-bonding interactions. The difference in molecular mechanism leads to photo-controlled Gram-selectivity and enhanced antibacterial activity. We found strain-specific and high bactericidal activity (minimal bactericidal concentration, 0.65 μg/ml) with low cytotoxicity, which we extended to wound healing applications. This methodology provides a single platform for efficiently switching between conformers to reversibly control the strain-selective bactericidal activity regulated by light. © 2023 Wiley-VCH GmbH.

Item Type:	Journal Article
Publication:	Angewandte Chemie - International Edition
Publisher:	John Wiley and Sons Inc
Additional Information:	The copyright for this article belongs to the publisher
Keywords:	Bacteria; Bactericides; Cytology; Hydrogen bonds; Image enhancement; Layered semiconductors, Anti-bacterial activity; Antibacterial therapy; Antibacterials; Biointerfaces; Gram-negative bacteria; Gram-positive bacterium; Gram-selectivity; Photoswitching; Single platform; Wound healing, Molybdenum compounds
Department/Centre:	Division of Biological Sciences > Biochemistry Division of Chemical Sciences > Materials Research Centre Division of Chemical Sciences > Organic Chemistry
Date Deposited:	01 Mar 2024 05:46
Last Modified:	01 Mar 2024 05:46
URI:	https://eprints.iisc.ac.in/id/eprint/83830

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