Observations on phenomenological changes in Klebsiella Pneumoniae under fluidic stresses

Jain, S and Singh, A and Tiwari, N and Naik, A and Chatterjee, R and Chakravortty, D and Basu, S (2023) Observations on phenomenological changes in Klebsiella Pneumoniae under fluidic stresses. In: Soft Matter, 9 (47). pp. 9239-9253.

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Abstract

In the present work, experiments are conducted to understand the consequence of stresses generated by flowing fluid on the bacterial morphology and virulence in microfluidic channels. We consider Klebsiella pneumoniae (KP, a clinical isolate), an ESKAPE pathogen, to be the model bacteria responsible for blood stream infections, bacteremia, including pneumonia, urinary tract infections and more. Four different stress conditions are generated by changing the flow rate and channel geometry subsequently altering the shear rate and stressing time (�). We observe significant changes in the structural aspects of the stressed bacteria. With an increase in stressing parameters, the viability of the bacterial sample deteriorated. Most importantly, these stressed samples proliferate much more than unstressed samples inside the RAW264.7 murine macrophages. The results shed light on the complex relationship between flow stresses and bacterial virulence. Furthermore, the bacterial samples are challenged with ciprofloxacin to see how they behave under different stress conditions. The observations presented in the present study can be extended to model deadly diseases including bacteremia using organ-on-a-chip technology and to understand bacterial pathogenicity under realistic environments. © 2023 The Royal Society of Chemistry.

Item Type:	Journal Article
Publication:	Soft Matter
Publisher:	Royal Society of Chemistry
Additional Information:	The copyright for this article belongs to Royal Society of Chemistry.
Keywords:	Bacteriology; Shear flow, Bacterial morphology; Blood streams; Channel geometry; Clinical isolates; Flowing fluid; Klebsiella pneumoniae; Microfluidics channels; Shear-rate; Stress condition; Urinary tract infections, Bacteria, antiinfective agent, animal; bacteremia; Klebsiella infection; Klebsiella pneumoniae; microbiology; mouse, Animals; Anti-Bacterial Agents; Bacteremia; Klebsiella Infections; Klebsiella pneumoniae; Mice
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology Division of Mechanical Sciences > Mechanical Engineering
Date Deposited:	01 Mar 2024 05:26
Last Modified:	01 Mar 2024 05:26
URI:	https://eprints.iisc.ac.in/id/eprint/83777

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