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ITPK1 Regulates Jasmonate-Controlled Root Development in Arabidopsis thaliana

Pullagurla, NJ and Shome, S and Yadav, R and Laha, D (2023) ITPK1 Regulates Jasmonate-Controlled Root Development in Arabidopsis thaliana. In: Biomolecules, 13 (9).

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Official URL: https://doi.org/10.3390/biom13091368


Jasmonic acid (JA) is a plant hormone that regulates a plethora of physiological processes including immunity and development and is perceived by the F-Box protein, Coronatine-insensitive protein 1 (COI1). The discovery of inositol phosphates (InsPs) in the COI1 receptor complex highlights their role in JAperception. InsPs are phosphate-rich signaling molecules that control many aspects of plant physiology. Inositol pyrophosphates (PP-InsPs) are diphosphate containing InsP species, of which InsP7 and InsP8 are the best characterized ones. Different InsP and PP-InsP species are linked with JA-related plant immunity. However, role of PP-InsP species in regulating JA-dependent developmental processes are poorly understood. Recent identification of ITPK1 kinase, responsible for the production of 5-InsP7 from InsP6 in planta, provides a platform to investigate the possible involvement of ITPK-derived InsP species in JA-related plant development. Here, in this study, we report that ITPK1-defective plants exhibit increased root growth inhibition to bioactive JA treatment. The itpk1 plants also show increased lateral root density when treated with JA. Notably, JA treatment does not increase ITPK1 protein levels. Gene expression analyses revealed that JA-biosynthetic genes are not differentially expressed in ITPK1-deficient plants. We further demonstrate that genes encoding different JAZ repressor proteins are severely down-regulated in ITPK1-defective plants. Taken together, our study highlights the role of ITPK1 in regulating JA-dependent root architecture development through controlling the expression of different JAZ repressor proteins. © 2023 by the authors.

Item Type: Journal Article
Publication: Biomolecules
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
Additional Information: The copyright for this article belongs to the Authors.
Keywords: inositol phosphate; jasmonic acid; jasmonic acid methyl ester, Arabidopsis thaliana; Article; biosynthesis; Bradford assay; controlled study; down regulation; electroblotting; gene; gene expression level; gene expression profiling; genotype; immunoblotting; itpk1 gene; lateral root; nonhuman; plant development; plant growth; plant immunity; polyacrylamide gel electrophoresis; protein expression; protein function; protein phosphorylation; real time polymerase chain reaction; RNA extraction; root development; root length; seedling; signal transduction; Western blotting
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 01 Dec 2023 04:32
Last Modified: 01 Dec 2023 04:32
URI: https://eprints.iisc.ac.in/id/eprint/83438

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