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A double-negative feedback loop between miR319c and JAW-TCPs establishes growth pattern in incipient leaf primordia in Arabidopsis thaliana

Shankar, N and Sunkara, P and Nath, U (2023) A double-negative feedback loop between miR319c and JAW-TCPs establishes growth pattern in incipient leaf primordia in Arabidopsis thaliana. In: PLoS Genetics, 19 (9).

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Official URL: https://doi.org/10.1371/journal.pgen.1010978


The microRNA miR319 and its target JAW-TCP transcription factors regulate the proliferation-to-differentiation transition of leaf pavement cells in diverse plant species. In young Arabidopsis leaf primordia, JAW-TCPs are detected towards the distal region whereas the major mRNA319-encoding gene MIR319C, is expressed at the base. Little is known about how this complementary expression pattern of MIR319C and JAW-TCPs is generated. Here, we show that MIR319C is initially expressed uniformly throughout the incipient primordia and is later abruptly down-regulated at the distal region, with concomitant distal appearance of JAW-TCPs, when leaves grow to ~100 μm long. Loss of JAW-TCPs causes distal extension of the MIR319C expression domain, whereas ectopic TCP activity restricts MIR319C more proximally. JAW-TCPs are recruited to and are capable of depositing histone H3K27me3 repressive marks on the MIR319C chromatin. JAW-TCPs fail to repress MIR319C in transgenic seedlings where the TCP-binding cis-elements on MIR319C are mutated, causing miR319 gain-of-function-like phenotype in the embryonic leaves. Based on these results, we propose a model for growth patterning in leaf primordia wherein MIR319C and JAW-TCPs repress each other and divide the uniformly growing primordia into distal differentiation zone and proximal proliferation domain. © 2023 Shankar et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Item Type: Journal Article
Publication: PLoS Genetics
Publisher: Public Library of Science
Additional Information: The copyright for this article belongs to the Authors.
Keywords: beta glucuronidase; dexamethasone; JAW-TCP; microRNA; miR319c; transcription factor; unclassified drug, Agrobacterium tumefaciens; Arabidopsis thaliana; Article; chromatin; chromatin immunoprecipitation; fungus growth; gel mobility shift assay; gene expression; genotype; genotyping; negative feedback; nonhuman; phenotype; plant growth; polymerase chain reaction; real time polymerase chain reaction; RNA isolation; transgene; yeast
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 01 Dec 2023 10:17
Last Modified: 01 Dec 2023 10:17
URI: https://eprints.iisc.ac.in/id/eprint/83406

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