Isolation and characterization of dengue protease inhibitory prenylated chalcone from Polyalthia cerasoides Stem bark

Mangala, KJ and Usha, BM and Ravikumar, YS (2021) Isolation and characterization of dengue protease inhibitory prenylated chalcone from Polyalthia cerasoides Stem bark. In: Research Journal of Biotechnology, 16 (3). pp. 62-67.

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Abstract

Dengue infection is one of the leading causes of death globally. Still today there are no therapeutic agents to treat the disease. Plants are rich source of therapeutic phytochemicals. In the present study, we have explored Polyalthia cerasoides for possible anti-dengue phytoconstituents. Dried powdered Polyalthia cerasoides stem bark was extracted with methanol. Then, the methanol extract was fractionated with petroleum ether. The petroleum ether fraction was chromatographed over silica gel column chromatography and eluted with petroleum ether and ethyl acetate. The compound isolated from fractions 8- 14 was characterized as 6, 4-dihydroxy-3-propane chalcone: a prenylated flavanone using mass spectroscopic data. The isolated prenylated flavanone was investigated for the inhibition of dengue viral protease using molecular docking studies and in vitro protease assay. Results indicated that the prenylated flavanone binds to the active site of dengue viral protease with binding energy -10.2 Kcal/mol and -12.8 Kcal/mol of Serotype 1 and Serotype 4 respectively. Further, the prenylated flavanone was investigated for the inhibition of DENV protease using in vitro high throughput protease assay. Results indicated that prenylated flavanone inhibits the DENV protease in a dose-dependent manner. Prenylated flavanone reported a less toxic effect against HepG2 cells in the MTT cytotoxicity test. Further investigations may lead to developing the isolated compound as a potential dengue viral therapeutic agent.

Item Type:	Journal Article
Publication:	Research Journal of Biotechnology
Publisher:	World Research Association
Additional Information:	The copyright for this article belongs to the World Research Association.
Keywords:	Antiviral; Chalcone; Dengue; Isolation; Polyalthia cerasoides
Department/Centre:	Division of Chemical Sciences > Organic Chemistry
Date Deposited:	30 Nov 2023 04:16
Last Modified:	30 Nov 2023 04:16
URI:	https://eprints.iisc.ac.in/id/eprint/82994

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