Behera, P and Singh, KK and Pandit, S and Saha, D and Saini, DK and De, M (2021) Machine Learning-Assisted Array-Based Detection of Proteins in Serum Using Functionalized MoS2Nanosheets and Green Fluorescent Protein Conjugates. In: ACS Applied Nano Materials, 4 (4). pp. 3843-3851.
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Abstract
Abnormal concentrations of a specific protein or the presence of some biomarker proteins may indicate life-threatening diseases. Pattern-based detection of specific analytes using affinity-regulated receptors is one of the potential alternatives to specific antigen-antibody-based detection. In this report, we have schemed a sensor array by using various functionalized two-dimensional (2D)-MoS2 nanosheets and green fluorescent protein (GFP) as the receptor and the signal transducer, respectively. Two-dimensional MoS2 has been used as a promising candidate for recognition of the bioanalytes because of its high surface-to-volume ratio compared to those of other nanomaterials. Easy surface tunability of this material provides additional advantages to analyze the target of interest. The optimized 2D-MoS2-GFP conjugates are able to discriminate 15 different proteins at 50 nM concentration with a detection limit of 1 nM. Moreover, proteins in the binary mixture and in the presence of serum were discriminated successfully. Ten different proteins in serum media at relevant concentrations were classified successfully with 100% jackknifed classification accuracy, which proves the potentiality of the above system. We have also implemented and discussed the implication of using different machine learning models on the pattern recognition problem associated with array-based sensing.
| Item Type: | Journal Article |
|---|---|
| Publication: | ACS Applied Nano Materials |
| Publisher: | American Chemical Society |
| Additional Information: | The copyright for this article belongs to the Author. |
| Keywords: | array-based sensing; machine learning; pattern recognition; protein sensing; two-dimensional MoS2 |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Chemical Sciences > Organic Chemistry |
| Date Deposited: | 07 Aug 2023 06:11 |
| Last Modified: | 07 Aug 2023 06:11 |
| URI: | https://eprints.iisc.ac.in/id/eprint/82829 |
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