Chandra, K and Al-Harthi, S and Sukumaran, S and Almulhim, F and Emwas, A-H and Atreya, HS and Jaremko, Ł and Jaremko, M (2021) NMR-based metabolomics with enhanced sensitivity. In: RSC Advances, 11 (15). pp. 8694-8700.
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Abstract
NMR-based metabolomics, which emerged along with mass spectrometry techniques, is the preferred method for studying metabolites in medical research and food industries. However, NMR techniques suffer from inherently low sensitivity, regardless of their superior reproducibility. To overcome this, we made two beneficial modifications: we detuned the probe to reach a position called “Spin Noise Tuning Optimum” (SNTO), and we replaced the conventional cylindrical 5 mm NMR tube with an electric field component-optimized shaped tube. We found that concerted use of both modifications can increase the sensitivity (signal to noise ratio per unit volume) and detection of metabolites and decrease the measurement time by order of magnitude. In this study, we demonstrate and discuss the achieved signal enhancement of metabolites on model non-human (bovine serum, amino acid standard mixture) and human urine samples.
| Item Type: | Journal Article |
|---|---|
| Publication: | RSC Advances |
| Publisher: | Royal Society of Chemistry |
| Additional Information: | The copyright for this article belongs to the author. |
| Keywords: | Biomolecules; Electric fields; Mammals; Mass spectrometry; Signal to noise ratio, Electric field components; Enhanced sensitivity; Human urine samples; Measurement time; Medical research; Reproducibilities; Signal enhancement; Spectrometry technique, Metabolites |
| Department/Centre: | Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility) |
| Date Deposited: | 17 Aug 2023 08:59 |
| Last Modified: | 17 Aug 2023 08:59 |
| URI: | https://eprints.iisc.ac.in/id/eprint/82803 |
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