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Transient association between proteins elicits alteration of dynamics at sites far away from interfaces

Tandon, H and de Brevern, AG and Srinivasan, N (2021) Transient association between proteins elicits alteration of dynamics at sites far away from interfaces. In: Structure, 29 (4). 371-384.e3.

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Official URL: https://doi.org/10.1016/j.str.2020.11.015


Tandon et al. perform systematic analyses on 3D structures of proteins available in free form and bound to another protein. They observe that protein-protein association leads to change in dynamics at sites away from interfaces, alters inter-residue communication, and affects the global motions accessible to the protein in its unbound form. © 2020 Elsevier Ltd Proteins are known to undergo structural changes upon binding to partner proteins. However, the prevalence, extent, location, and function of change in protein dynamics due to transient protein-protein interactions is not well documented. Here, we have analyzed a dataset of 58 protein-protein complexes of known three-dimensional structure and structures of their corresponding unbound forms to evaluate dynamics changes induced by binding. Fifty-five percent of cases showed significant dynamics change away from the interfaces. This change is not always accompanied by an observed structural change. Binding of protein partner is found to alter inter-residue communication within the tertiary structure in about 90% of cases. Also, residue motions accessible to proteins in unbound form were not always maintained in the bound form. Further analyses revealed functional roles for the distant site where dynamics change was observed. Overall, the results presented here strongly suggest that alteration of protein dynamics due to binding of a partner protein commonly occurs.

Item Type: Journal Article
Publication: Structure
Publisher: Cell Press
Additional Information: The copyright for this article belongs to the author.
Keywords: allostery; bioinformatics; functional analysis; normal mode analysis; protein dynamics; protein structure; protein-protein interactions
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 17 Aug 2023 09:17
Last Modified: 17 Aug 2023 09:17
URI: https://eprints.iisc.ac.in/id/eprint/82802

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