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Dual Functionalized Injectable Hybrid Extracellular Matrix Hydrogel for Burn Wounds

Bankoti, K and Rameshbabu, AP and Datta, S and Goswami, P and Roy, M and Das, D and Ghosh, SK and Das, AK and Mitra, A and Pal, S and Maulik, D and Su, B and Ghosh, P and Basu, B and Dhara, S (2021) Dual Functionalized Injectable Hybrid Extracellular Matrix Hydrogel for Burn Wounds. In: Biomacromolecules, 22 (2). pp. 514-533.

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Official URL: https://doi.org/10.1021/acs.biomac.0c01400


Low strength and rapid biodegradability of acellular dermal matrix (ADM) restrict its wider clinical application as a rapid cell delivery platform in situ for management of burn wounds. Herein, the extracted ADM was modified by a dual cross-linking approach with ionic crosslinking using chitosan and covalent cross-linking using an iodine-modified 2,5-dihydro-2,5-dimethoxy-furan cross-linker, termed as CsADM-Cl. In addition, inherent growth factors and cytokines were found to be preserved in CsADM-Cl, irrespective of ionic/covalent crosslinking. CsADM-Cl demonstrated improvement in post crosslinking stiffness with a decreased biodegradation rate. This hybrid crosslinked hydrogel supported adhesion, proliferation, and migration of human foreskin-derived fibroblasts and keratinocytes. Also, the angiogenic potential of CsADM-Cl was manifested by chick chorioallantoic membrane assay. CsADM-Cl showed excellent antibacterial activity against Escherichia coli and Staphylococcus aureus. Moreover, CsADM-Cl treated full thickness burn wounds and demonstrated rapid healing marked with superior angiogenesis, well-defined dermal-epidermal junctions, mature basket weave collagen deposition, and development of more pronounced secondary appendages. Altogether, the bioactive CsADM-Cl hydrogel established significant clinical potential to support wound healing as an apt injectable antibacterial matrix to encounter unmet challenges concerning critical burn wounds.

Item Type: Journal Article
Publication: Biomacromolecules
Publisher: American Chemical Society
Additional Information: The copyright for this article belongs to the Author.
Keywords: Biodegradability; Biodegradation; Cell culture; Escherichia coli; Hydrogels; Tissue regeneration, Acellular dermal matrixes; Anti-bacterial activity; Chick chorioallantoic membrane; Clinical application; Covalent crosslinking; Cross-linked hydrogels; Dermal-epidermal junctions; Extracellular matrices, Crosslinking, chitosan; hydrogel, adult; angiogenesis; animal experiment; animal model; animal tissue; antibacterial activity; antioxidant activity; Article; biodegradation; burn; cell adhesion; cell migration; cell proliferation; chorioallantoic membrane assay; controlled study; covalent bond; cross linking; dermoepidermal junction; drug efficacy; Escherichia coli; extracellular matrix; fibroblast; in vitro study; in vivo study; keratinocyte; nonhuman; priority journal; rat; Staphylococcus aureus; wound healing; acellular dermal matrix; burn; extracellular matrix; human; hydrogel, Acellular Dermis; Burns; Extracellular Matrix; Humans; Hydrogels; Wound Healing
Department/Centre: Division of Chemical Sciences > Materials Research Centre
Date Deposited: 28 Jul 2023 09:18
Last Modified: 28 Jul 2023 09:18
URI: https://eprints.iisc.ac.in/id/eprint/82678

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