Utilization of DNA and 2D metal oxide interaction for an optical biosensor

Kumbhakar, P and Das Jana, I and Basu, S and Mandal, S and Banerjee, S and Roy, S and Gowda, CC and Chakraborty, A and Pramanik, A and Lahiri, P and Lahiri, B and Chandra, A and Kumbhakar, P and Mondal, A and Maiti, PK and Tiwary, CS (2023) Utilization of DNA and 2D metal oxide interaction for an optical biosensor. In: Physical Chemistry Chemical Physics .

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Abstract

The efficient monitoring and early detection of viruses may provide essential information about diseases. In this work, we have highlighted the interaction between DNA and a two-dimensional (2D) metal oxide for developing biosensors for further detection of viral infections. Spectroscopic measurements have been used to probe the efficient interactions between single-stranded DNA (ssDNA) and the 2D metal oxide and make them ideal candidates for detecting viral infections. We have also used fully atomistic molecular dynamics (MD) simulation to give a microscopic understanding of the experimentally observed ssDNA-metal oxide interaction. The adsorption of ssDNA on the inorganic surface was found to be driven by favourable enthalpy change, and 5′-guanine was identified as the interacting nucleotide base. Additionally, the in silico assessment of the conformational changes of the ssDNA chain during the adsorption process was also performed in a quantitative manner. Finally, we comment on the practical implications of these developments for sensing that could help design advanced systems for preventing virus-related pandemics.

Item Type:	Journal Article
Publication:	Physical Chemistry Chemical Physics
Publisher:	Royal Society of Chemistry
Additional Information:	The copyright for this article belongs to the Royal Society of Chemistry.
Keywords:	Biosensors; Metallic compounds; Molecular dynamics; Viruses, 2D metal; Detection of virus; Efficient interaction; Efficient monitoring; Metal-oxide; Metal-oxide interactions; Optical bio-sensors; Spectroscopic measurements; Two-dimensional; Viral infections, DNA
Department/Centre:	Division of Physical & Mathematical Sciences > Physics
Date Deposited:	24 Jul 2023 12:19
Last Modified:	24 Jul 2023 12:19
URI:	https://eprints.iisc.ac.in/id/eprint/82625

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