Monolayer protein adsorption on cationic gold nanoparticles: A thermodynamic study

Saikia, S and Das, PK (2023) Monolayer protein adsorption on cationic gold nanoparticles: A thermodynamic study. In: Chemical Physics Letters, 825 .

PDF che_phy_let_825_2023.pdf - Published Version Restricted to Registered users only Download (2MB) | Request a copy

Abstract

The mechanism for adsorption of Lysozyme (Lyz) and cytochrome c (Cyt. c) proteins on cetyltrimethylammonium bromide (CTAB) capped cationic gold nanoparticles (GNPs) of 26, 46, and 68 nm at pH 6.8 were probed by second harmonic light scattering technique (SHLS). We found that both the proteins binds with cationic GNPs with surprisingly high binding constant of 109-1010 M−1. The nsat values clearly indicates that both the proteins form only monolayer on the GNP surface. Dynamic light scattering measurements validate the adsorption parameters obtained from SHLS. The temperature-dependent SHLS measurements revealed that the adsorption processes were favoured by huge entropic gain.

Item Type:	Journal Article
Publication:	Chemical Physics Letters
Publisher:	Elsevier B.V.
Additional Information:	The copyright for this article belongs to the Elsevier B.V.
Keywords:	Adsorption; Entropy change; Free energy change; Gold nanoparticles; Protein
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	09 Jul 2023 17:34
Last Modified:	09 Jul 2023 17:34
URI:	https://eprints.iisc.ac.in/id/eprint/82082

Actions (login required)

View Item