Acetylcholine Structure-Based Small Activatable Fluorogenic Probe for Specific Detection of Acetylcholinesterase

Sidhu, JS and Rajendran, K and Mathew, AB and Iqbal, T and Saini, DK and Das, D (2023) Acetylcholine Structure-Based Small Activatable Fluorogenic Probe for Specific Detection of Acetylcholinesterase. In: Analytical Chemistry .

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Abstract

Early detection of Alzheimer’s disease (AD) is important for taking proper measures against AD pathogenesis. Acetylcholinesterase (AChE) is widely reported to be associated with the pathogenicity of AD. Here, employing the “acetylcholine-mimic” approach, we designed and synthesized a new class of naphthalimide (Naph)-based fluorogenic probes for specific detection of AChE and avoiding interference of butyrylcholinesterase (BuChE), the pseudocholinesterase. We investigated the action of the probes on Electrophorus electricus AChE, and the native human brain AChE that we expressed in Escherichia coli and purified in the active form for the first time. The probe Naph-3 exhibited a substantial fluorescence enhancement with AChE and majorly avoided BuChE. Naph-3 successfully crossed the cell membrane of the Neuro-2a cells and fluoresced upon reaction with endogenous AChE. We further established that the probe could be effectively used for screening AChE inhibitors. Our study provides a new avenue for the specific detection of AChE, which can be extended to the diagnosis of AChE-related complications.

Item Type:	Journal Article
Publication:	Analytical Chemistry
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to American Chemical Society.
Keywords:	Cytology; Diagnosis; Escherichia coli, Acetylcholinesterase; Alzheimer; Butyrylcholinesterase; Fluorogenic probes; Human brain; Naphthalimide; Pseudocholinesterase; Specific detection; Structure-based; Synthesised, Probes
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry Others
Date Deposited:	15 Jun 2023 10:19
Last Modified:	15 Jun 2023 10:19
URI:	https://eprints.iisc.ac.in/id/eprint/82058

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